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Occurrence, diagnosis and follow-up of canine strongyloidiosis in naturally infected shelter dogs

Published online by Cambridge University Press:  30 July 2018

Roberta Iatta
Dipartimento di Medicina Veterinaria, Università degli Studi di Bari, Valenzano, 70010 Bari, Italy
Dora Buonfrate
Centre for Tropical Diseases, IRCCS Sacro Cuore Don Calabria Hospital, Negrar, Verona, Italy
Paola Paradies
Dipartimento dell'Emergenza e dei Trapianti di organi, Sezione di Veterinaria, Università degli Studi di Bari, Valenzano, 70010 Bari, Italy
Maria Alfonsa Cavalera
Dipartimento di Medicina Veterinaria, Università degli Studi di Bari, Valenzano, 70010 Bari, Italy
Antonio Capogna
Dipartimento dell'Emergenza e dei Trapianti di organi, Sezione di Veterinaria, Università degli Studi di Bari, Valenzano, 70010 Bari, Italy
Fabrizio Iarussi
Dipartimento dell'Emergenza e dei Trapianti di organi, Sezione di Veterinaria, Università degli Studi di Bari, Valenzano, 70010 Bari, Italy
Jan Šlapeta
Dipartimento di Medicina Veterinaria, Università degli Studi di Bari, Valenzano, 70010 Bari, Italy Sydney School of Veterinary Science, Faculty of Science, University of Sydney, Sydney, NSW, 2006, Australia
Giovanni Giorli
Centre for Tropical Diseases, IRCCS Sacro Cuore Don Calabria Hospital, Negrar, Verona, Italy
Paolo Trerotoli
Dipartimento di Scienze Biomediche e Oncologia Umana, Università degli Studi di Bari, 70100 Bari, Italy
Zeno Bisoffi
Centre for Tropical Diseases, IRCCS Sacro Cuore Don Calabria Hospital, Negrar, Verona, Italy Dipartimento di Diagnostica e Sanità Pubblica, Università degli Studi di Verona, Verona, Italy
Domenico Otranto*
Dipartimento di Medicina Veterinaria, Università degli Studi di Bari, Valenzano, 70010 Bari, Italy
Author for correspondence: Domenico Otranto, E-mail:


Strongyloidiosis by Strongyloides stercoralis is a disease of increasing interest in human and animal medicine. The scientific knowledge on canine strongyloidiosis is hindered by the poor diagnostics available. To assess the most sensitive and specific diagnostic method, feces and blood from 100 shelter dogs were screened for S. stercoralis by coprological, molecular and serological tests. Thirty-six dogs (36%) scored positive to S. stercoralis by coprology (22.3% to Baermann) and/or 30% to real time-polymerase chain reaction (rt-PCR). According to two composite reference standards (CRS) based on all coprological methods and rt-PCR (first CRS) or in combination with serology (second CRS), the most sensitive test was IFAT (93.8%; CI 82.8–98.7), followed by rt-PCR (80.6%; 95% CI 64–91.8) and Baermann (60.6%; 95% CI 42.1–77.1). The inconsistent shedding of L1 during the 4-week follow-up in infected dogs suggests the importance of multiple faecal collections for a reliable diagnosis. A combination of serological and coprological tests is recommended for the surveillance and diagnosis of S. stercoralis infection in dogs.

Research Article
Copyright © Cambridge University Press 2018 

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