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Antagonistic activity of the fungus Pochonia chlamydosporia on mature and immature Toxocara canis eggs

Published online by Cambridge University Press:  23 March 2012

A. S. MACIEL ,
L. G. FREITAS ,
L. D. FIGUEIREDO ,
A. K. CAMPOS  and
I. N. K. MELLO
A. S. MACIEL*
Affiliation:
Laboratório de Controle Biológico de Nematóides, Instituto de Biotecnologia Aplicada à Agropecuária, Universidade Federal de Viçosa, Minas Gerais, 36570-000, Brazil
L. G. FREITAS
Affiliation:
Laboratório de Controle Biológico de Nematóides, Instituto de Biotecnologia Aplicada à Agropecuária, Universidade Federal de Viçosa, Minas Gerais, 36570-000, Brazil
L. D. FIGUEIREDO
Affiliation:
Laboratório de Controle Biológico de Nematóides, Instituto de Biotecnologia Aplicada à Agropecuária, Universidade Federal de Viçosa, Minas Gerais, 36570-000, Brazil
A. K. CAMPOS
Affiliation:
Instituto de Ciências da Saúde, Universidade Federal do Mato Grosso, Mato Grosso, 78550-000, Brazil
I. N. K. MELLO
Affiliation:
Laboratório de Parasitologia, Departamento de Veterinária, Universidade Federal de Viçosa, Minas Gerais, 36570-000, Brazil
*
*Corresponding author: Laboratório de Controle Biológico de Nematóides, Bioagro, Universidade Federal de Viçosa, Minas Gerais, 36570-000, Brazil. Tel: +55 31 38992925. E-mail: ale_spalenza@yahoo.com.br
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Summary

In vitro tests were performed to evaluate the ability of 6 isolates of the nematophagous fungus Pochonia chlamydosporia to infect immature and mature Toxocara canis eggs on cellulose dialysis membrane. There was a direct relationship between the number of eggs colonized and the increase in the days of interaction, as well as between the number of eggs colonized and the increase in the concentration of chlamydospores (P<0·05). Immature eggs were more susceptible to infection than mature eggs. The isolate Pc-04 was the most efficient egg parasite until the 7th day, and showed no difference in capacity to infect mature and immature eggs in comparison to Pc-07 at 14 and 21 days of interaction, respectively. Isolate Pc-04 was the most infective on the two evolutionary phases of the eggs at most concentrations, but its ability to infect immature eggs did not differ from that presented by the isolates Pc-07 and Pc-10 at the inoculum level of 5000 chlamydospores. Colonization of infective larvae inside or outside the egg was observed in treatments with the isolates Pc-03, Pc-04, Pc-07 and Pc-10. The isolate Pc-04 of P. chlamydosporia has great biological capacity to destroy immature and mature T. canis eggs in laboratory conditions.

Keywords

Toxocara canisPochonia chlamydosporianematophagous fungibiological controldogs
Type
Research Article
Information
Parasitology , Volume 139 , Issue 8 , July 2012 , pp. 1074 - 1085
Copyright
Copyright © Cambridge University Press 2012

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