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Molecular investigation of genet distribution and genetic variation of Cortinarius rotundisporus in eastern Australian sclerophyll forests

Published online by Cambridge University Press:  01 June 1999

N. A. SAWYER ,
S. M. CHAMBERS  and
J. W. G. CAIRNEY
N. A. SAWYER
Affiliation:
Mycorrhiza Research Group, School of Science, University of Western Sydney (Nepean), PO Box 10, Kingswood, NSW 2747, Australia
S. M. CHAMBERS
Affiliation:
Mycorrhiza Research Group, School of Science, University of Western Sydney (Nepean), PO Box 10, Kingswood, NSW 2747, Australia
J. W. G. CAIRNEY
Affiliation:
Mycorrhiza Research Group, School of Science, University of Western Sydney (Nepean), PO Box 10, Kingswood, NSW 2747, Australia
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Abstract

The size and distribution of Cortinarius rotundisporus genets at three sclerophyll forest field sites in New South Wales, Australia, were estimated by using microsatellite-primed PCR (MS-PCR) of DNA extracted from sporocarp tissue. MS-PCR fingerprints generated with the primers (GTG)5 and (GACA)4 indicated that two to five genets were present at each site, with each site being characterized by a single large genet (9–30 m in diameter). Analysis of internal transcribed spacer (ITS)-RFLP patterns from individual sporocarps used in the study suggested that three distinct RFLP types were present in the sampled C. rotundisporus population. ITS sequence data indicate that the three RFLP types had less than 88.4% sequence identity to each other, strongly suggesting that C. rotundisporus is a complex of three species.

Keywords

ectomycorrhizal fungimicrosatellite-primed PCRinternal transcribed spacer (ITS) sequencesITS-RFLP
Type
Research Article
Information
The New Phytologist , Volume 142 , Issue 3 , June 1999 , pp. 561 - 568
Copyright
© Trustees of the New Phytologist 1999

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