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Oligodendrocytes regulate formation of nodes of Ranvier via the recognition molecule OMgp

Published online by Cambridge University Press:  25 July 2006

DU-YU NIE
Affiliation:
Institute of Molecular and Cell Biology, Singapore Department of Anatomy, National University of Singapore, Singapore
QUAN-HONG MA
Affiliation:
Institute of Molecular and Cell Biology, Singapore Sino-Germany Center for Neuroscience, Dalian Medical University, Dalian, China
JANICE W.S. LAW
Affiliation:
Department of Clinical Research, Singapore General Hospital, Singapore
CHERN-PANG CHIA
Affiliation:
Department of Clinical Research, Singapore General Hospital, Singapore
NARENDER K. DHINGRA
Affiliation:
Department of Clinical Research, Singapore General Hospital, Singapore
YASUSHI SHIMODA
Affiliation:
Department of Bioengineering, Nagaoka University of Technology, Nagaoka, Japan
WU-LIN YANG
Affiliation:
Department of Clinical Research, Singapore General Hospital, Singapore
NENG GONG
Affiliation:
Institute of Neuroscience, Shanghai, China
QING-WEN CHEN
Affiliation:
Department of Clinical Research, Singapore General Hospital, Singapore National Neuroscience Institute, Singapore
GANG XU
Affiliation:
Department of Clinical Research, Singapore General Hospital, Singapore
QI-DONG HU
Affiliation:
Institute of Molecular and Cell Biology, Singapore Department of Anatomy, National University of Singapore, Singapore
PIERCE K.H. CHOW
Affiliation:
Department of Experimental Surgery, Singapore General Hospital, Singapore
YEE-KONG NG
Affiliation:
Department of Anatomy, National University of Singapore, Singapore
ENG-ANG LING
Affiliation:
Department of Anatomy, National University of Singapore, Singapore
KAZUTADA WATANABE
Affiliation:
Department of Bioengineering, Nagaoka University of Technology, Nagaoka, Japan
TIAN-LE XU
Affiliation:
Institute of Neuroscience, Shanghai, China
AMYN A. HABIB
Affiliation:
Department of Neurology, University of Texas Southwestern Medical Center, Dallas, USA
MELITTA SCHACHNER
Affiliation:
Sino-Germany Center for Neuroscience, Dalian Medical University, Dalian, China Zentrum fur Molekulare Neurobiologie, University of Hamburg, Hamburg, Germany
ZHI-CHENG XIAO
Affiliation:
Institute of Molecular and Cell Biology, Singapore Department of Anatomy, National University of Singapore, Singapore Department of Clinical Research, Singapore General Hospital, Singapore

Abstract

The molecular mechanisms underlying the involvement of oligodendrocytes in formation of the nodes of Ranvier (NORs) remain poorly understood. Here we show that oligodendrocyte-myelin glycoprotein (OMgp) aggregates specifically at NORs. Nodal location of OMgp does not occur along demyelinated axons of either Shiverer or proteolipid protein (PLP) transgenic mice. Over-expression of OMgp in OLN-93 cells facilitates process outgrowth. In transgenic mice in which expression of OMgp is down-regulated, myelin thickness declines, and lateral oligodendrocyte loops at the node-paranode junction are less compacted and even join together with the opposite loops, which leads to shortened nodal gaps. Notably, each of these structural abnormalities plus modest down-regulation of expression of Na+ channel α subunit result in reduced conduction velocity in the spinal cords of the mutant mice. Thus, OMgp that is derived from glia has distinct roles in regulating nodal formation and function during CNS myelination.

Type
Article
Copyright
Cambridge University Press 2006

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