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Diversity and vertical transmission of double-stranded RNA elements in root rot pathogens of trees, Helicobasidium mompa and Rosellinia necatrix

Published online by Cambridge University Press:  14 June 2004

Ken-ichi IKEDA ,
Hitoshi NAKAMURA ,
Masao ARAKAWA  and
Naoyuki MATSUMOTO
Ken-ichi IKEDA
Affiliation:
National Institute for Agro-Environmental Sciences, 3-1-3 Kan-non dai, Tsukuba 305-8604, Japan. E-mail: nowmat@affrc.go.jp
Hitoshi NAKAMURA
Affiliation:
National Institute for Agro-Environmental Sciences, 3-1-3 Kan-non dai, Tsukuba 305-8604, Japan. E-mail: nowmat@affrc.go.jp Present address: National Institute of Fruit Tree Science 2-1 Fujimoto, Tsukuba 305-8605, Japan.
Masao ARAKAWA
Affiliation:
National Institute for Agro-Environmental Sciences, 3-1-3 Kan-non dai, Tsukuba 305-8604, Japan. E-mail: nowmat@affrc.go.jp Present address: Faculty of Agriculture, Meijo University, Nagoya, 468-8502, Japan.
Naoyuki MATSUMOTO
Affiliation:
National Institute for Agro-Environmental Sciences, 3-1-3 Kan-non dai, Tsukuba 305-8604, Japan. E-mail: nowmat@affrc.go.jp
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Abstract

The diversity and vertical transmission of double-stranded (ds) RNA in Helicobasidium mompa and Rosellinia necatrix was examined by electrophoresis and Northern hybridization. These two fungi share the similar niche as root rot pathogens of trees in forests and orchards, and had diverse dsRNAs. The detection frequency of dsRNA in both fungi was different; in H. mompa, 68.4% (132 out of 193 MCGs; mycelial compatibility groups) had dsRNA, whereas 20.9% (53 out of 254 MCGs) in R. necatrix. dsRNA banding patterns and Northern blot analyses revealed the presence of various dsRNA elements in both fungi. Hyphal tip isolation was mostly unsuccessful to remove dsRNA with some exceptions. Sexual reproduction functioned to remove dsRNA in both fungi since dsRNA was not detected from single sexual spore cultures. Possible explanations for the difference in the detection frequency of dsRNA are discussed in terms of the differences in their sexual reproduction and other factors.

Type
Research Article
Information
Mycological Research , Volume 108 , Issue 6 , June 2004 , pp. 626 - 634
Copyright
© The British Mycological Society 2004

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