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Phytophthora ramorum sp. nov., a new pathogen on Rhododendron and Viburnum

Published online by Cambridge University Press:  28 November 2001

Sabine Werres
Affiliation:
Federal Biological Research Centre, Institute for Plant Virology, Microbiology and Biosafety – Department of Microbiology, Königin-Luise-Straße 19, D-14195 Berlin, Germany.
Rainer Marwitz
Affiliation:
Federal Biological Research Centre, Institute for Plant Virology, Microbiology and Biosafety – Department of Microbiology, Königin-Luise-Straße 19, D-14195 Berlin, Germany.
Willem A. Man In't Veld
Affiliation:
Plantenziektenkundige Dienst, Postbus 9102, 6700 HC Wageningen, The Netherlands.
Arthur W. A. M. De Cock
Affiliation:
Centraalbureau voor Schimmelcultures (CBS), P.O. Box 85167, 3508 AD Utrecht, The Netherlands.
Peter J. M. Bonants
Affiliation:
Plant Research International, P.O. Box 16, 6700 AA Wageningen, The Netherlands. E-mail: s.werres@bba.de
Marjanne De Weerdt
Affiliation:
Plant Research International, P.O. Box 16, 6700 AA Wageningen, The Netherlands. E-mail: s.werres@bba.de
Karin Themann
Affiliation:
Federal Biological Research Centre, Institute for Plant Protection in Horticulture, Messeweg 11/12, D-38104 Braunschweig, Germany.
Elena Ilieva
Affiliation:
Plantenziektenkundige Dienst, Postbus 9102, 6700 HC Wageningen, The Netherlands.
Robert P. Baayen
Affiliation:
Plantenziektenkundige Dienst, Postbus 9102, 6700 HC Wageningen, The Netherlands.
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Abstract

Since 1993, a hitherto unidentified Phytophthora species has been found associated with twig blight disease in Rhododendron and, sporadically, Viburnum. The morphology and growth characteristics of fourteen isolates from Germany and the Netherlands were investigated, together with their breeding system, the internal transcribed spacer (ITS) regions of the ribosomal DNA, amplified fragment length polymorphism (AFLP) fingerprints, and isozyme profiles, which were compared to those of a number of outgroup species. Morphologically the isolates are characterized by abundant production of chlamydospores and elongate, ellipsoid, deciduous sporangia with a short pedicel, in which they resemble P. palmivora. However, sporangia were semi-papillate, chlamydospores were much larger and cardinal temperatures much lower than those of P. palmivora. Oogonia with amphigynous antheridia and plerotic oospores were produced in dual cultures with an A2 mating type strain of P. cryptogea. ITS1 and ITS2 sequences of the unidentified species were closest to those of P. lateralis, but differed in three and eight nucleotides respectively from the latter species. AFLP fingerprints and isozyme patterns of malate dehydrogenase (MDH-2) and malic enzyme (MDHP) showed that the isolates formed a homogeneous group, distinct from all examined outgroup species, including P. lateralis. It was concluded that they represent a new Phytophthora species, described here as P. ramorum sp. nov. In pathogenicity tests all isolates of P. ramorum were pathogenic to Rhododendron.

Type
Research Article
Copyright
Copyright © The British Mycological Society 2001

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