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Uncoiling of DNA by Double Chained Cationic Surfactants

Published online by Cambridge University Press:  10 February 2011

H. H. Paradies  and
S. F. Clancy
H. H. Paradies
Affiliation:
Biotechnology & Physical Chemistry, Märkische Fachhochschule, Iserlohn, D- 58644, Germany, HParadies@aol.com
S. F. Clancy
Affiliation:
Witco Corporation, Safety, Health, & Environmental Affairs, One American Lane, Greenwich, CT 06831–2559, USA, clancsh@witco-corp.com
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Abstract

Distearyldimethylammonium (DSDMA) X (X− = OH, H2PO4) interact with double stranded T4 DNA (166 kilobase pairs) below and above the CMC (1.5×10−6). Below the CMC of either DSDMA X where the cationic double-chained surfactants are in the monomeric state, T4 DNA and DSDMA+form a compact complex where all surfactant molecules are bound. Close to the CMC, particularly for DSDMA OH but also in the presence of , T4 DNA exhibits a condensed (globule) conformation, though some DNA molecules are in the more extended DNA conformation. Above the CMC of DSDMA X a plateau is reached (up to 3.0 × 10−6 M surfactant) revealing a penetration of DSDMA molecules into the DNA globules resulting in a loosening of the tightened state of DNA. The various stages of the transition from the condensed coil to the extended state of T4 DNA with changing DSDMA X concentrations was monitored by static and inelastic light scattering experiments which were supplemented by small-angle X-ray scattering measurements.

Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 463: Symposia EE – Statistical Mechanics in Physics and Biology , 1996 , 49
Copyright
Copyright © Materials Research Society 1997

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