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Three-Dimensional PEG Hydrogel Construct Fabrication using Stereolithography

Published online by Cambridge University Press:  01 February 2011

Karina Arcaute ,
Luis Ochoa ,
Frank Medina ,
Chris Elkins ,
Brenda Mann  and
Ryan Wicker
Karina Arcaute
Affiliation:
W.M. Keck Border Biomedical Manufacturing and Engineering Laboratory, Mechanical Engineering, University of Texas at El Paso, El Paso, TX, USA.
Luis Ochoa
Affiliation:
W.M. Keck Border Biomedical Manufacturing and Engineering Laboratory, Mechanical Engineering, University of Texas at El Paso, El Paso, TX, USA.
Frank Medina
Affiliation:
W.M. Keck Border Biomedical Manufacturing and Engineering Laboratory, Mechanical Engineering, University of Texas at El Paso, El Paso, TX, USA.
Chris Elkins
Affiliation:
Mechanical Engineering and Radiology, Stanford University, Stanford, CA, USA.
Brenda Mann
Affiliation:
Sentrx Surgical, Inc., Salt Lake City, UT, USA.
Ryan Wicker
Affiliation:
W.M. Keck Border Biomedical Manufacturing and Engineering Laboratory, Mechanical Engineering, University of Texas at El Paso, El Paso, TX, USA.
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Abstract

Layered manufacturing (LM) using stereolithography (SL) of aqueous polymer solutions was accomplished so three-dimensional (3D) tissue engineered scaffolds with complex distributions of bioactive agents could be produced. Successful LM with embedded channel architectures required investigation of hydrogel thickness or cure depth as a function of photoinitiator type and concentration, energy dosage, and polymer concentration in solution. Poly(ethylene glycol) dimethacrylate (PEG-dma) with an average molecular weight of 1000 in quantities of 20% and 30% (w/v) was prepared in distilled water. Different concentrations of two photoinitiators (PIs), Sarcure1121 (2-hydroxy-2-methyl-1-phenyl-1-propanone) and Irgacure 2959 (2-hydroxy-1-[4-(2-hydroxyethoxy)phenyl]-2-methyl-1-propanone), were used to vary gel thickness at select energy dosages by controlling the scan speed of the SL machine's ultraviolet scanning system. Gel thickness was a strong function of PI type and concentration, energy dosage, and PEG-dma concentration, especially at the low PI concentrations required for implantation. The gel thickness curves were utilized to demonstrate LM for two construct geometries where different layer thicknesses were required to successfully fabricate the constructs. This work demonstrates the effective use of SL as a processing technique for complex 3D tissue scaffolds and addresses some practical considerations associated with the use of hydrogels in LM.

Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 874: Symposium l – Structure and Mechanical Behavior of Biological Materials , 2005 , L5.5
Copyright
Copyright © Materials Research Society 2005

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References

1. Lu, L. and Mikos, A.G., MRS Bulletin, 21(11), 28–32 (1996).Google Scholar
2. Ma, P.X., Materials Today, 7(5), 30–40 (2004).Google Scholar
3. Comeau, B.M., Umar, Y., Gonsalves, K.E. and Henderson, C.L., MRS Symp.Proc. 845, (2005).Google Scholar
4. Cooke, M.N., Fisher, J.P., Dean, D., Rimnac, C. and Mikos, A.G., J. Biomed Mater Res. 64B(2), 65–69 (2003).Google Scholar
5. Dhariwala, B., Hunt, E. and Boland, T., Tissue Engineering, 10(9/10), 1316–1322 (2004).Google Scholar
6. Harris, J. Milton & Zalipsky, Samuel, Poly (ethylene glycol): Chemistry and Biological Applications ACS Symposium Series 680. (ACS, Washington, DC, 1997).Google Scholar
7. Mann, B.K., Schmedlen, R.H., West, J.L., Biomaterials, 22, 439–44 (2001).Google Scholar
8. Mann, B.K., Gobin, A.S., Tsai, A.T., Schmedlen, R.H., West, J.L., Biomaterials 22, 3045–51 (2001).Google Scholar
9. Leach, J.B., Bivens, K.A., Patrick, C.W. and Schmidt, C.E., Biotechnology and Bioengineering, 82(5), 578–589 (2003).Google Scholar
10. Bryant, S.J., Nuttelman, C.R. and Anseth, K.S., J. Biomater. Sci. Polymer Edn, 11(5), 439–457 (2000).Google Scholar