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Piezoelectric Poly(3-hydroxybutyrate)-Poly(lactic acid) Three Dimensional Scaffolds for Bone Tissue Engineering

Published online by Cambridge University Press:  01 February 2011

Juana Mendenhall ,
Dapeng Li ,
Margaret Frey ,
Juan Hinestroza ,
Omotunde Babalola ,
Lawrence Bonnasar  and
Carl A Batt
Juana Mendenhall
Affiliation:
juana.mendenhall@bme.gatech.edu, GA Tech, Biomedical Engineering, 313 Ferst Drive Room 1221, Atlanta, GA, 30332, United States, 404-668-3197
Dapeng Li
Affiliation:
dl325@cornell.edu, Cornell University, Fiber Science and Apparel Design, Ithaca, NY, 14853, United States
Margaret Frey
Affiliation:
mfw24@cornell.edu, Cornell University, Fiber Science and Apparel Design, Ithaca, NY, 14853, United States
Juan Hinestroza
Affiliation:
jh433@cornell.edu, Cornell University, Fiber Science and Apparel Design, Ithaca, NY, 14853, United States
Omotunde Babalola
Affiliation:
omb3@cornell.edu, Cornell University, Biomedical Engineering, Ithaca, NY, 14853, United States
Lawrence Bonnasar
Affiliation:
lb244@cornell.edu, Cornell University, Biomedical Engineering, Ithaca, NY, 14853, United States
Carl A Batt
Affiliation:
cab10@cornell.edu, Cornell University, Food Science, Ithaca, NY, 14853, United States
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Abstract

Three dimensional scaffolds (3D) are promising for future nanoscale materials and tissue engineering applications being that they have architecture and mechanical properties similar to natural tissue. In this work, poly(lactic acid) fibers were prepared via electrospinnig with average diameters of 2580 nm. Using Enzymatic Surface-Initiated polymerization (ESIP), poly(3-hydroxybutyrate) were coated on poly(lactic acid) fibers. This provides an alternative method to enzymatic surface modification of fibers. ESIP of PHB produces a granular film providing surface topography and increases mechanical properties of PLA fibers alone. When using covalent approaches, PHB granules provide surface topography of 200-500 nm with a polydisperse coverage area. Compressive modulus measurements of PLA and PHB/PLA scaffolds were 25 kPa and 73 kPa, respectively. The percent crystallinity of PLA and PHB/PLA scaffolds was 17% and 30%, respectively. This rough topography, in addition to the crystallinity of the scaffold, facilitates Soas-2 osteoblast cell attachment. We have observed attachment of the osteoblast cells along the length of the oriented PLA and PHB/PLA composite scaffolds with different morphologies, rounded and stretched, throughout a depth of 90 µm.

Keywords

biomaterialfibertissue
Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 1025: Symposium B – Nanoscale Phenomena in Functional Materials by Scanning Probe Microscopy , 2007 , 1025-B12-03
Copyright
Copyright © Materials Research Society 2008

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