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Nano-scale Debye Capacitive Sensors for Highly Sensitive, Label-free, Nucleic Acid Analysis

Published online by Cambridge University Press:  01 February 2011

Manu Sebastian Mannoor ,
Teena James ,
Dentcho V. Ivanov ,
Bill Braunlin  and
Les Beadling
Manu Sebastian Mannoor
Affiliation:
msm28@njit.edumanusmannoor@gmail.com, NJIT, Microelectronics Research Center, Newark, New Jersey, United States
Teena James
Affiliation:
tj35@njit.edu, NJIT, Microelectronics Research Center, Newark, New Jersey, United States
Dentcho V. Ivanov
Affiliation:
ivanov@njit.edu, NJIT, Microelectronics Research Center, Newark, New Jersey, United States
Bill Braunlin
Affiliation:
bbraunlin@rationalaffinity.com, Rational Affinity Devices LLC, Newark, New Jersey, United States
Les Beadling
Affiliation:
lbeadling@rationalaffinity.com, Rational Affinity Devices LLC, United States
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Abstract

An electrochemical capacitive sensor with electrode separation in the order of the Electrical Double Layer width (Debye length) of the analyte solution is presented for extremely sensitive and label-free analysis of Nucleic Acids. As the Electrical Double Layers (EDL) from both the capacitive electrodes interact and overlap each other in the reduced space confinement, the contribution from the electrode polarization effects and noises due to bulk sample resistance are found to be minimized. The dielectric property changes during hybridization reaction were measured using 10-mer nucleotide sequences. A 30-45% change in relative permittivity (capacitance) was observed due to DNA hybridization at 10Hz.

Keywords

nanoscalemicroelectro-mechanical (MEMS)biomedical
Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 1138: Symposium FF – Nanofunctional Materials, Structures and Devices for Biomedical Applications , 2008 , 1138-FF06-01
Copyright
Copyright © Materials Research Society 2009

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References

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