Hostname: page-component-7c8c6479df-8mjnm Total loading time: 0 Render date: 2024-03-29T15:21:50.206Z Has data issue: false hasContentIssue false

Nanoparticle-Decorated Surfaces for the Study of Cell-Protein-Substrate Interactions

Published online by Cambridge University Press:  01 February 2011

Jake D. Ballard
Affiliation:
Materials Science & Engineering, Rensselaer Polytechnic Institute, Troy, NY, USA. Rensselaer Nanotechnology Center, Rensselaer Polytechnic Institute, Troy, NY, USA.
Ludovico M. Dell'Acqua-Bellavitis
Affiliation:
Engineering Science, Rensselaer Polytechnic Institute, Troy, NY, USA. Rensselaer Nanotechnology Center, Rensselaer Polytechnic Institute, Troy, NY, USA.
Rena Bizios
Affiliation:
Biomedical Engineering, Rensselaer Polytechnic Institute, Troy, NY, USA. Rensselaer Nanotechnology Center, Rensselaer Polytechnic Institute, Troy, NY, USA.
Richard W. Siegel
Affiliation:
Materials Science & Engineering, Rensselaer Polytechnic Institute, Troy, NY, USA. Rensselaer Nanotechnology Center, Rensselaer Polytechnic Institute, Troy, NY, USA.
Get access

Abstract

The present study was motivated by the need for accurately-controlled and well-characterized novel biomaterial formulations for the study of cell-protein-material interactions. For this purpose, the current research has focused on the design, fabrication and characterization of model native oxide-coated silicon surfaces decorated with silica nanoparticles of select sizes, and has examined the adhesion of osteoblasts and fibroblasts on these nanoparticle-decorated surfaces. The results demonstrate the capability to deposit nanoparticles of select diameters and substrate surface coverage onto native silicon oxide-coated silicon, the firm attachment of these nanoparticles to the underlying native silicon oxide, and that nanoparticle size and coverage modulate adhesion of osteoblasts and fibroblasts to these substrates. The material formulations tested provide a well-controlled and well-characterized set of model substrates needed to study the effects of nanoscale features on the functions of cells that are critical to the clinical fate of implantable biomaterials.

Type
Research Article
Copyright
Copyright © Materials Research Society 2005

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

1. Webster, T. J., Schadler, L. S., Siegel, R. W., and Bizios, R., Tissue Engineering 7, 291 (2001).Google Scholar
2. McManus, A.J., Doremus, R. H., Siegel, R.W., and Bizios, R., J. Biomed. Mat. Res. (Published Online 10 November 2004).Google Scholar
3. Webster, T. J., Ergun, C., Doremus, R. H., Siegel, R. W., and Bizios, R., J. Biomed. Mat. Res. 51, 475 (2000).Google Scholar
4. Puleo, D. A., Holleran, L. H., Doremus, R. H., Bizios, R., J. Biomed. Mat. Res. 25, 711 (1991).Google Scholar