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Nanoindentation of Histological Specimens using an Extension of the Oliver and Pharr Method

Published online by Cambridge University Press:  01 February 2011

Riaz Akhtar ,
Michael J Sherratt ,
Nick Bierwisch ,
Brian Derby ,
Paul M Mummery ,
Rachel E.B Watson  and
Norbert Schwarzer
Riaz Akhtar
Affiliation:
riaz.akhtar@manchester.ac.uk, The University of Manchester, Manchester Materials Science Centre, School of Materials,Grosvenor Street, Manchester, M1 7HS, United Kingdom
Michael J Sherratt
Affiliation:
michael.j.sherratt@manchester.ac.uk, The University of Manchester, Tissue Injury and Repair Group, Faculty of Medical and Human Sciences, 1.581 Stopford Building, Oxford Road, Manchester, M13 9PT, United Kingdom
Nick Bierwisch
Affiliation:
n.bierwisch@siomec.de, Saxonian Institute of Surface Mechanics (SIO), Tankow 1, Ummanz, 18569, Germany
Brian Derby
Affiliation:
brian.derby@manchester.ac.uk, The University of Manchester, Manchester Materials Science Centre, School of Materials, Grosvenor Street, Manchester, M1 7HS, United Kingdom
Paul M Mummery
Affiliation:
paul.mummery@manchester.ac.uk, The University of Manchester, Manchester Materials Science Centre, School of Materials, Grosvenor Street, Manchester, M1 7HS, United Kingdom
Rachel E.B Watson
Affiliation:
rachel.watson@manchester.ac.uk, The University of Manchester, Dermatological Sciences Research Group, Faculty of Medical and Human Sciences, 1.443 Stopford Building, Oxford Road, Manchester, M13 9PT, United Kingdom
Norbert Schwarzer
Affiliation:
n.schwarzer@siomec.de, Saxonian Institute of Surface Mechanics (SIO), Tankow 1, Ummanz, 18569, Germany
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Abstract

The micro-mechanical properties of 5 μm thick histological sections of ferret aorta and vena cava were mapped as a function of distance from the outer adventitial layer using nanoindentation. In order to decouple the effect of the glass substrate on the elastic modulus of these thin sections, the nanoindentation data were analyzed using the extended Oliver and Pharr method which is readily accessible for coatings and layered materials with the software package, FilmDoctor®. In the aorta, the elastic modulus was found to decrease progressively from 35 MPa at the adventitia (outermost layer) to 8 MPa at the intima (innermost layer). This decrease in modulus was inversely correlated with elastic fibre density. In contrast, in the vena cava, the stiffest regions were found to be the adventitial (outer) and intimal (innermost) sections of the vessel cross-section. Both these regions were enriched in ECM components. The central region, thought to be largely cellular, had a relatively constant modulus of around 20 MPa. This study demonstrates that with this methodology it is possible to distinguish micro-mechanically between large arteries and veins, and therefore the same approach should allow age or disease related changes in the mechanical properties within a tissue to be quantified.

Keywords

nano-indentationtissueelastic properties
Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 1097: Symposium GG – Mechanical Behavior of Biological Materials and Biomaterials , 2008 , 1097-GG01-09
Copyright
Copyright © Materials Research Society 2008

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References

1 Bailey, A.J., Mech. Ageing Dev. 122, 735 (2001).Google Scholar
2 Kielty, C.M., Sherratt, M.J. and Shuttleworth, C.A., J. Cell. Sci. 115, 2817 (2002).Google Scholar
3 Cattell, M.A., Anderson, J.C. and Hasleton, P.S., Clinica Chimica Acta, 245, 73 (1996).Google Scholar
4 Escolar, J.D., Tejero, C., Escolar, M.A., Montalvo, F. and Garisa, R., Anat. Rec., 63 248, (1997).Google Scholar
5 Glasser, S.P., Arnett, D.K., McVeigh, G.E., Finkelstein, S.M., Bank, A.J., Morgan, D.J., and Cohn, J.N., Amer. J. Hyper., 10, 1175 (1997).Google Scholar
6 Aoun, S., Blacher, J., Safar, M.E., and Mourad, J.J., J. Hum. Hyper. 15, 693 (2001).Google Scholar
7 Boutouyrie, P., Tropeano, A.I., Asmar, R., Gautier, I., Benetos, A., Lacolley, P., and Laurent, S., Hyper. 39, 10 (2002).Google Scholar
8 Cruickshank, K., Riste, L., Anderson, S.G., Wright, J.S., Dunn, G., Circulation., 106, 2085 (2002)Google Scholar
9 Kimoto, E., Shoji, T.,Shinohara, K., Hatsuda, S., Mori, K.,Fukumoto, S., Koyama, H., Emoto, M., Okuno, Y., and Nishizawa, Y., J. Amer. Soc. Nephr. 17, 2245 (2006).Google Scholar
10 Sherratt, M.J., Bastrilles, J.Y., Bowden, J., Watson, R.E.B., and Griffiths, C.E.M., Brit.J. Derm. 155, 240 (2006)Google Scholar
11 Ebenstein, D.M. and Pruitt, L.A., J. Biomed. Mater. Res. 69A, 222 (2004).Google Scholar
12 Schwarzer, N., J. Phys. D: Appl. Phys., 37, 2761 (2004).Google Scholar
13 Oliver, W.C. and Pharr, G.M., J. Mater. Res. 7, 1564 (1992).Google Scholar
14 Chudoba, T. and Herrmann, K., Härtereitechnische Mitteilungen. HTM 56, 258 (2001).Google Scholar
15 Bolshakov, A., Oliver, W.C., and Pharr, G.M., MRS Symp. Proc. 356, 675 (1995).Google Scholar
16 Pharr, G.M. and Bolshakov, A., J. Mater. Res., 17, 2660 (2002).Google Scholar
17 Schwarzer, N., and Pharr, G. M., Th. Sol. Films. 469-470C, 194 (2004).Google Scholar
18 Schwarzer, N., Chudoba, T., and Pharr, G. M., Surf. Coat. Technol., 200, 4220 (2006).Google Scholar
19 Schwarzer, N., Chudoba, T., and Richter, F., Surf. Coat. Technol. 200, 5566 (2006).Google Scholar
20 Schwarzer, N., Th. So. Fims, 494, 168 (2006).Google Scholar
21 Schwarzer, N., Phil. Mag. 86, 5153 (2006).Google Scholar
22 Keeley, F.W., Bellingham, C.M. and Woodhouse, K.A., Phil Trans Roy Soc ser B. 357, 185 (2002).Google Scholar
23 Yang, L., van der Werf, K.O., Koopman, B.F.J.M., Subramaniam, V., Bennink, M.L., Dijkstra, P.J., and Feijen, J., J Biomed Mater Res A. 82A, 160 (2007).Google Scholar