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Multilayer Enzyme Assembly for the Development of a Novel Fiber Optic Biosensor

Published online by Cambridge University Press:  15 February 2011

Harry H. Gao ,
Zhongping Chen ,
Jayant Kumar ,
Kenneth A. Marx ,
Sukant K. Tripathy  and
David L. Kaplan
Harry H. Gao
Affiliation:
Center for Advanced Materials, Departments of Physics
Zhongping Chen
Affiliation:
Chemistry, University of Massachusetts Lowell, Lowell, MA. 01854, kumarj@woods.uml.edu Biotechnology Division, US Army Natick RD&E Center, Natick, MA. 01760
Jayant Kumar
Affiliation:
Center for Advanced Materials, Departments of Physics
Kenneth A. Marx
Affiliation:
Chemistry, University of Massachusetts Lowell, Lowell, MA. 01854, kumarj@woods.uml.edu
Sukant K. Tripathy
Affiliation:
Chemistry, University of Massachusetts Lowell, Lowell, MA. 01854, kumarj@woods.uml.edu
David L. Kaplan
Affiliation:
Biotechnology Division, US Army Natick RD&E Center, Natick, MA. 01760
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Abstract

We have developed a technique to immobilize a multilayer enzyme assembly on an optical fiber surface. The technique utilizes a bifunctional amino coupling agent to link between different layers of enzymes. The method offers the flexibility of controlling the number of enzymes on a fiber surface. Multilayers of enzyme — Alkaline Phosphatase have been characterized using ellipsometry and surface plasma resonance. Chemiluminecence experiments have been conducted as a function of number of enzyme layers. The results indicated that at least 3 layers of enzyme can be assembled on a fiber surface. Utilising the new immobilization technique, a novel fiber optic biosensor system for the detection of organophosphorous-based pesticide has been developed. The detection mechanism is the pesticide inhibition of alkaline phosphatase catalyzed chemiluminescence. As a model analyte, paraoxon with ppb level concentration has been detected.

Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 414: Symposium Z – Thin Films and Surfaces for Bioactivity and Biomedical Applications , 1995 , 125
Copyright
Copyright © Materials Research Society 1996

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References

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