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Micropillar array embedded system for single cell encapsulation in hydrogel

Published online by Cambridge University Press:  09 February 2015

Kyun Joo Park ,
Kyoung G. Lee ,
Seunghwan Seok ,
Bong Gill Choi ,
Seok Jae Lee  and
Do Hyun Kim
Kyun Joo Park
Affiliation:
Department of Chemical & Biomolecular Engineering, KAIST, Korea.
Kyoung G. Lee
Affiliation:
Center for Nanobio Integration & Convergence Engineering (NICE), National Nanofab Center, Korea.
Seunghwan Seok
Affiliation:
Department of Chemical & Biomolecular Engineering, KAIST, Korea.
Bong Gill Choi
Affiliation:
Department of Chemical Engineering, Kangwon National University, Korea.
Seok Jae Lee
Affiliation:
Center for Nanobio Integration & Convergence Engineering (NICE), National Nanofab Center, Korea.
Do Hyun Kim*
Affiliation:
Department of Chemical & Biomolecular Engineering, KAIST, Korea.
*
*Email: Dohyun.kim@kaist.ac.kr
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Abstract

A cylindrical-shaped micropillar array embedded microfluidic device was proposed to enhance the dispersion of cell clusters and the efficiency of single cell encapsulation in hydrogel. Different sizes of micropillar arrays act as a sieve to break Escherichia coli (E. coli) aggregates into single cells in polyethylene glycol diacrylate (PEGDA) solution. We applied the external force for the continuous breakup of cell clusters, resulting in the production of more than 70% of single cells into individual hydrogel particles. This proposed strategy and device will be a useful platform to utilize genetically modified microorganisms in practical applications.

Keywords

biomaterialfluidicsmicrostructure
Type
Articles
Information
MRS Online Proceedings Library (OPL) , Volume 1724: Symposium H – Micro/Nano Engineering and Devices for Molecular and Cellular Manipulation, Simulation and Analysis , 2014 , mrsf14-1724-h12-03
Copyright
Copyright © Materials Research Society 2015 

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References

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