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Metal-Enhanced Fluorescence of Chlorophylls in Single Light-Harvesting Complexes

Published online by Cambridge University Press:  31 January 2011

Sebastian Mackowski ,
Dawid Piatkowski ,
Stephan Wörmke ,
Achim Hartschuh ,
Christoph Bräeuchle ,
Tatas H. P. Brotosudarmo ,
Hugo Scheer ,
Ashish Agarwal ,
Nicholas A. Kotov  and
Alexander O Govorov
Sebastian Mackowski
Affiliation:
mackowski@fizyka.umk.pl, Nicolaus Copernicus University, Institute of Physics, Torun, Poland
Dawid Piatkowski
Affiliation:
dapi@fizyka.umk.pl, Nicolaus Copernicus University, Institute of Physics, Torun, Poland
Stephan Wörmke
Affiliation:
woermke@googlemail.com, Ludwig-Maximilian-University, Department of Chemistry and Biochemistry and Center for Nanoscience, Munich, Germany
Achim Hartschuh
Affiliation:
achim.hartschuh@cup.uni-muenchen.de, Ludwig-Maximilian-University, Department of Chemistry and Biochemistry and Center for Nanoscience, Munich, Germany
Christoph Bräeuchle
Affiliation:
Christoph.Braeuchle@cup.uni-muenchen.de
Tatas H. P. Brotosudarmo
Affiliation:
t.brotosudarmo.1@research.gla.ac.uk, Ludwig-Maximilian-University, Department of Biology, Munich, Germany
Hugo Scheer
Affiliation:
H.Scheer@biologie.uni-muenchen.de, Ludwig-Maximilian-University, Department of Biology, Munich, Germany
Ashish Agarwal
Affiliation:
agashish@umich.edu, University of Michigan, Department of Chemical Engineering, Ann Arbor, Michigan, United States
Nicholas A. Kotov
Affiliation:
kotov@umich.edu, United States
Alexander O Govorov
Affiliation:
govorov@sholoarone.com, Ohio University, Department of Physics and Astronomy, Athens, Ohio, United States
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Abstract

We show that the fluorescence of peridinin-chlorophyll a-protein complexes can be strongly enhanced via coupling with plasmon excitations localized in metal nanostructures. The results of ensemble and single-molecule spectroscopy experiments at room temperature demonstrate six-fold increase of the emission intensity of the light-harvesting complex when it is placed in the vicinity of chemically prepared silver islands. Irrespective of the enhancement, we observe no effect of the metal nanoparticle on the fluorescence emission energy of the complex. This observation implies that plasmon excitations may be applied for controlling the optical properties of complex biomolecules.

Keywords

Agbiologicalluminescence
Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 1208: Symposium O – Excitons and Plasmon Resonances in Nanostructures II , 2009 , 1208-O14-01
Copyright
Copyright © Materials Research Society 2010

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