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Inelastic Neutron Scattering Studies of Natural Silkworm Proteins

Published online by Cambridge University Press:  30 June 2015

Christopher Alan Crain ,
Nicholas A. Strange  and
J. Z. Larese
Christopher Alan Crain
Affiliation:
University of Tennessee, Knoxville, Tennessee, United States
Nicholas A. Strange
Affiliation:
University of Tennessee, Knoxville, Tennessee, United States
J. Z. Larese
Affiliation:
University of Tennessee, Knoxville, Tennessee, United States
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Abstract

Understanding the interaction of water with biological materials is of fundamental importance. One of main driving forces behind the renewed activity of biomimetic materials involves the dramatic physical properties that many of them exhibit. Two main factors that are critical for understanding silks: the nanoscale semi-crystalline folding structure, and the degree of hydration of the disordered fraction. We describe our investigation of the preparation, characterization and inelastic neutron scattering (INS) studies of the microscopic dynamics of natural Bombyx mori silk fibroin proteins derived from silkworm cocoons. An in situ quartz microbalance is used for monitoring/controlling the hydration and solvent levels of the proteins electrospun onto neutron sample holders. By employing these novel methods our INS investigation facilitated a snapshot of the microscopic silk protein dynamics heretofore not investigated or reported. Preliminary INS measurements illustrate the effect of water and methanol interaction on the dynamics of the fibroin β-pleated sheet component. Evidence of what appears to be a water component (intersheet) distinct from bulk water is clearly apparent in the INS spectrum when the dynamical response from the dry silk is subtracted away.

Keywords

biomaterialneutron scatteringadsorption
Type
Articles
Information
MRS Online Proceedings Library (OPL) , Volume 1793: Symposium GG – Foundations of Bio/Nano Interfaces―Synthesis, Modeling, Design Principles and Applications , 2015 , pp. 41 - 46
Copyright
Copyright © Materials Research Society 2015 

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