Hostname: page-component-8448b6f56d-tj2md Total loading time: 0 Render date: 2024-04-24T20:49:24.197Z Has data issue: false hasContentIssue false
  • English
  • Français

Highly Efficient Detector of the Neurotransmitter ACh and AChE Inhibitors

Published online by Cambridge University Press:  15 March 2011

Ilya Goykhman ,
Nina Korbakov ,
Carmen Bartic ,
Gustaaf Borghs ,
Micha E. Spira ,
Josef Shappir  and
Shlomo Yitzchaik
Ilya Goykhman
Affiliation:
Department of Analytical and Inorganic Chemistry, The Hebrew University of Jerusalem, Jerusalem, 91904, Israel School of Engineering, The Hebrew University of Jerusalem, Jerusalem, 91904, Israel
Nina Korbakov
Affiliation:
Department of Analytical and Inorganic Chemistry, The Hebrew University of Jerusalem, Jerusalem, 91904, Israel
Carmen Bartic
Affiliation:
IMEC, Kapeldreef 75, Leuven, B-3001, Belgium
Gustaaf Borghs
Affiliation:
IMEC, Kapeldreef 75, Leuven, B-3001, Belgium
Micha E. Spira
Affiliation:
Department of Neurobiology, The Hebrew University of Jerusalem, Jerusalem, 91904, Israel
Josef Shappir
Affiliation:
School of Engineering, The Hebrew University of Jerusalem, Jerusalem, 91904, Israel
Shlomo Yitzchaik
Affiliation:
Department of Analytical and Inorganic Chemistry, The Hebrew University of Jerusalem, Jerusalem, 91904, Israel
Get access

Abstract

Among the variety of applications for biosensorsone of the exciting frontiers is to utilize those devices as post-synaptic sensing elements in chemical coupling between neurons and solid-state systems. The first necessary step to attain this challenge is to realize highly efficient detector for neurotransmitter acetylcholine (ACh). Herein, we demonstrate that the combination of floating gate configuration of ion-sensitive field effect transistor (ISFET) together with diluted covalent anchoring of enzyme acetylcholinesterase (AChE) onto device sensing area reveals a remarkable improvement of a four orders of magnitude in dose response to ACh. This high range sensitivity in addition to the benefits of peculiar microelectronic design show, that the presented hybrid provides a competent platform for assembly of artificial chemical synapse junction. Furthermore, our system exhibits clear response to eserine, a competitive inhibitor of AChE, and therefore it can be implemented as an effective sensor of pharmacological reagents, organophosphates, and nerve gases as well.

Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 1009: Symposium U – Advanced Materials for Neuroprosthetic Interfaces , 2007 , 1009-U08-04
Copyright
Copyright © Materials Research Society 2007

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

1. Kharitonov, A.B., Zayats, M., Lichtenstein, A., Katz, E., Willner, I., Sens. Actuators B, 70, 222–231 (2000).Google Scholar
2. Bergveld, P., Sens. Actuators B, 88, 1–20 (2003).Google Scholar
3. Hai, A., Ben-Haim, D., Korbakov, N., Cohen, A., Shappir, J., Oren, R., Spira, M.E., Yitzchaik, S., Bioelectronics & Biosensors, 22(5), 605–612 (2006).Google Scholar
4. Cohen, A., Spira, M.E., Yitzchaik, S., Borghs, G., Shwartzglass, O., Shappir, J., Bioelectronics & Biosensors, 19(12), 1703–1709 (2004).Google Scholar
5. Poghossian, A., Schöning, M.J., Electroanalysis, 16, 1863–1872 (2004).Google Scholar
6. Schöning, M.J., Arzdorf, M., Mulchandani, P., Chen, W., Mulchandani, A., Sens. Actuators B, 91, 92–97 (2003).Google Scholar
7. Hara, H., Ohta, T., Sens. Actuators B, 32, 115–119 (1996).Google Scholar
8. Christensen, C., Reus, R. de, Bouwstra, S., J. Micromech. Microeng., 9, 113–118 (1999).Google Scholar
9. Chaneliere, C.. Aurtan, J.L., Devine, R.A.B., Balland, B., Mater. Science. Eng., R22, 269–322 (1998).Google Scholar
10. Bergveld, P., Siabbald, A., Analytical and biomedical application of ion-selective field-effect transistors, Comprehensive Analytical chemistry, (Wilson and Wilson's), V. XXIII (1988).Google Scholar
11. Bhatia, S.K., Cooney, M.J., Shriver-Lake, L.C., Fare, T.L., Ligler, F.S., 1991. Sens.Actuators B 3:311.Google Scholar
12. Karnovsky, M.J., Roots, L., Jour. Histochem. & Cytochem., 12, 219 (1964).Google Scholar
13. Dewa, A.S., Ko, W.H. in Semiconductor Sensor, edited by Sze, S.M., (A Wiley-Interscience Publication, John Wiley & Sons, Inc., 605 Third Avenue, New York, 1994), pp. 415–472. Google Scholar
14. McMurray, W.C., Essentials of Human Metabolism. The Relationship of Biochemistry to Human Physiology and Disease, Medical Department Harper and Row, Publishers Hagerstown, Maryland, New York, San Francisco, London, 1977.Google Scholar