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Enhanced Detection of Cardiovascular Biomarker Proteins: A Detailed Study of Nanoconfinement in Nanoporous Membrane

Published online by Cambridge University Press:  19 March 2012

Savindra Brandigampala ,
Paige Feikert ,
Krishna Vattipalli  and
Shalini Prasad
Savindra Brandigampala
Affiliation:
Department of Electrical Engineering and Computer Science, Wichita State University, 1845 Fairmount St, Wichita, KS 67260, U.S.A.
Paige Feikert
Affiliation:
Bio-Engineering Program, College of Engineering, Wichita State University, 1845 Fairmount St, Wichita, KS 67260, U.S.A.
Krishna Vattipalli
Affiliation:
Department of Electrical Engineering and Computer Science, Wichita State University, 1845 Fairmount St, Wichita, KS 67260, U.S.A.
Shalini Prasad
Affiliation:
Department of Electrical Engineering and Computer Science, Wichita State University, 1845 Fairmount St, Wichita, KS 67260, U.S.A. Bio-Engineering Program, College of Engineering, Wichita State University, 1845 Fairmount St, Wichita, KS 67260, U.S.A.
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Abstract

The goal of this work is to understand the role of nano-confinement in designing an inexpensive and user friendly ‘point- of- care’ (POC) protein biosensor. We used printed circuit board based gold chips and integrated them with nanoporous alumina membranes in generating high density arrays of nano scale confined spaces. We initially tested the role of a nanomembrane in achieving signal enhancement through size based confinement of proteins. As a later part of the experiment, we studied the role of pore size on achieving signal enhancement by using membranes of two different pore sizes of 100 and 200 nm. It is critical that ultralow detection of biomolecules be achieved as they have significant impact in designing diagnostics platforms for early disease diagnosis. Commercially available nano-porous membranes made out of anodized alumina were evaluated for their role in nano-confinement and enhancing sensitivity of detection. In this biosensor configuration sandwich assay, an electrical double layer is formed between a test protein (C-reactive protein) and the gold surface underneath the porous membrane. Using electrical impedance spectroscopy, the capacitance/impedance changes in the electrical double layer, was analyzed which translated to identifying the sensitivity and the linear dose response of the sensor for two specific conditions (a) with nano confinement and (b) for varying size of confined spaces

Keywords

nanostructuresensorbiomedical
Type
Articles
Information
MRS Online Proceedings Library (OPL) , Volume 1355: Symposium JJ – Biological Hybrid Materials for Life Sciences , 2011 , mrss12-1355-jj09-15
Copyright
Copyright © Materials Research Society 2012

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References

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