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Eels Imaging of Biological Materials

Published online by Cambridge University Press:  21 February 2011

R.D. Leapman ,
S. Sun ,
J.A. Hunt  and
S.B. Andrews
R.D. Leapman
Affiliation:
Biomedical Engineering and Instrumentation Program, National Center for Research Resources, National Institutes of Health, Bethesda, MD 20892
S. Sun
Affiliation:
Biomedical Engineering and Instrumentation Program, National Center for Research Resources, National Institutes of Health, Bethesda, MD 20892
J.A. Hunt
Affiliation:
Gatan R & D, Pleasanton, CA 94566
S.B. Andrews
Affiliation:
Laboratory of Neurobiology, National Institute for Neurological Diseases and Stroke National Institutes of Health, Bethesda, MD 20892
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Abstract

Parallel-detection electron energy loss spectroscopy (EELS) in the scanning transmission electron microscope provides a very sensitive means of detecting specific elements in biological systems. By analyzing EELS spectrum-image data recorded from rapidly-frozen and cryosectioned tissue it is possible to map quantitatively the distribution of the biologically important element, calcium, which is typically present at concentrations of only a few parts per million in cellular structures some tens of nanometers in diameter. A significant improvement (factor of four) in calcium detectability has been demonstrated for EELS compared with energy-dispersive x-ray spectroscopy. The spectrum-imaging technique has also been applied to map water distributions in hydrated biological specimens by utilizing the valence electron excitations.

Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 332: Symposium U – Determining Nanoscale Physical Properties of Materials by Microscopy and Spectroscopy , 1994 , 351
Copyright
Copyright © Materials Research Society 1994

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References

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