Hostname: page-component-8448b6f56d-jr42d Total loading time: 0 Render date: 2024-04-16T11:33:07.396Z Has data issue: false hasContentIssue false
  • English
  • Français

Development of Novel Nanostructured Tissue Engineering Scaffold Materials through Self-assembly for Bed-side Orthopedic Applications

Published online by Cambridge University Press:  01 February 2011

Lijie Zhang ,
Sharwatie Ramsaywack ,
Hicham Fenniri  and
Thomas J. Webster
Lijie Zhang
Affiliation:
Lijie_zhang@brown.edu, Brown Univeristy, Division of Engineering, 182 Hope street, Box D, Providence, RI, 02912, United States, 518-986-3335, 401-863-9107
Sharwatie Ramsaywack
Affiliation:
sr12@ualberta.ca, University of Alberta and National Institute for Nanotechnology, Department of Chemistry,, 11421 Saskatchewan Drive, Edmonton, AB T6G 2M9, Canada
Hicham Fenniri
Affiliation:
hicham.fenniri@ualberta.ca, University of Alberta and National Institute for Nanotechnology, Department of Chemistry,, 11421 Saskatchewan Drive, Edmonton, AB T6G 2M9, Canada
Thomas J. Webster
Affiliation:
Thomas_Webster@brown.edu, Brown Univeristy, Divisions of Engineering and Orthopaedics, 182 Hope Street,, Providence, RI, 02912, United States
Get access

Abstract

The objective of the current study was to utilize a natural self-assembled organic biomaterial (helical rosette nanotubes (HRNs)) to improve bone growth necessary for orthopedic implant applications. The DNA base pair building blocks of HRNs can self-assemble through 18 H-bonds to form a supermacrocycle in water which then stack to form a nanotube 3.5 nm in diameter and several μm in length. The nanometric features and ability to place diverse amino acid side chains on HRNs make them intriguing materials for orthopedic applications. In this study, HRNs are combined with a biocompatible hydrogel matrix in order to obtain more robust scaffolds. Bone cell experiments in vitro demonstrated that the novel HRNs with hydrogels could greatly enhance osteoblast (bone-forming cell) adhesion even at a very low concentration (close to 0.001mg/ml). Morphology of the HRNs with hydrogel scaffolds was characterized by transmission electron microscopy (TEM), scanning electron microscopy (SEM) and atomic force microscopy (AFM). Results showed that there were bundles of nanotubes in the HRNs with hydrogel scaffolds. Therefore, considering the good biocompatibility and nano bone-like structure of these scaffolds, the nanostructured hydrogel matrix with HRNs have the potential to serve as novel bone building agents for “on-the-site” orthopedic applications.

Keywords

bonenanostructurefracture
Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 950: Symposium D – Biosurfaces and Biointerfaces , 2006 , 0950-D15-08
Copyright
Copyright © Materials Research Society 2007

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

1. Kaplan, F. S., Hayes, W. C., Keaveny, T. M., Boskey, A., Einhorn, T. A., and Iannotti, J. P., Form and function of bone., Orthopedic Basic Science, ed. Sinmon, S. P., (American Academy of Orthopaedic Surgeons, 1994) pp.127–185.Google Scholar
2. Webster, T. J., .Nanophase ceramics: the future orthopedic and dental implant material., Advances in Chemical Engineering, ed. Ying, J. Y., (Academic Press, 2001), pp.125–166.Google Scholar
3. Webster, T. J., Siegel, R. W. and Bizios, R., Biomaterials 20, 1221 (1999).Google Scholar
4. Webster, T. J. and Ejiofor, J. U., Biomaterials 25, 4731 (2004).Google Scholar
5. Burg, K. J. L., Porter, S. and Kellam, J. F., Biomaterials 21, 2347 (2000).Google Scholar
6. Summers, B. N., Eisenstein, S. M., J. Bone Jt. Surg. Br. 71–B, 677 (1989).Google Scholar
7. Younger, E. M., Chapman, M. W., J. Orthop. Trauma 3, 192 (1989).Google Scholar
8. Fenniri, H., Mathivanan, P., Vidale, K. L., Sherman, D. M., Hallenga, K., Wood, K. V. and Stowell, J. G., J. Am. Chem. Soc. 123, 3854 (2001).Google Scholar
9. Chun, A. L., Moralez, J. G., Fenniri, H. and Webster, T. J., Nanotechnology 15, S234 (2004).Google Scholar
10. Chun, A. L., Moralez, J. G., Webster, T. J. and Fenniri, H., Biomaterials 26, 7304 (2005).Google Scholar
11. Lee, K.Y. and Mooney, D. J., Chemical Reviews 101, 1869 (2001).Google Scholar
12. Hoffman, A. S., Advanced Drug Delivery Reviews 43, 3 (2002).Google Scholar
13. Zavrel, V. and Štol, M., Biomaterials 14, 1109 (1993).Google Scholar
14. Cadotte, A. J. and DeMarse, T. B., J. Neural Eng. 2, 114 (2005).Google Scholar
15. Refojo, M. F. and Yasuda, H., J. Appl. Polym. Sci. 9, 2425 (1965).Google Scholar
16. Wisniewski, S. and Kim, S.W., J. Membr. Sc. 6, 309 (1980).Google Scholar
17. Zhou, H. Y., Ohnuma, Y., Takita, H., Fujisawa, R., Mizuno, M. and Kuboki, Y., Biochem. Biophys. Res. Commun. 186, 1288 (1992).Google Scholar
18. Dalton, P. D., Flynn, L., and Shoichet, M. S., Biomaterials 23 3843 (2002).Google Scholar
19. Song, J., Saiz, E. and Bertozzi, C. R., J. Am. Chem. Soc. 125, 1236 (2003).Google Scholar
20. Weiner, S. and Wagner, H. D., Annu. Rev. Mater. Sci. 28, 271 (1998).Google Scholar
21. Song, J., Saiz, E. and Bertozzi, C. R., J. Eur. Ceramic Soc. 23, 2905 (2003).Google Scholar