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Detection of Bacillus Anthracis Spores Using Magnetostrictive Microcantilever-based Biosensor

Published online by Cambridge University Press:  01 February 2011

Liling Fu ,
Suiqiong Li ,
Kewei Zhang  and
Z.-Y. Cheng
Liling Fu
Affiliation:
Auburn University, Auburn University, 275 Wilmore Labs,, Materials Research and Education Center, Auburn University, Auburn, AL, 36849, United States
Suiqiong Li
Affiliation:
lisuiqi@auburn.edu, Auburn University, Materials Research and Education Center, Auburn, AL, 36849, United States
Kewei Zhang
Affiliation:
zhangke@auburn.edu, Auburn University, Materials Research and Education Center, Auburn, AL, 36849, United States
Z.-Y. Cheng
Affiliation:
chengzh@eng.auburn.edu, Auburn University, Materials Research and Education Center, Auburn, AL, 36849, United States
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Abstract

Recently, the magnetostrictive microcantilever (MSMC) as a high performance biosensor platform was introduced. The MSMC is a wireless acoustic wave (AW) sensor and exhibits a high Q value. More importantly, the MSMC works well in liquid. In this paper, the detection of Bacillus anthracis spores using MSMCs with filamentous phage as the bioprobe is reported. The phased-coated MSMC biosensors were exposed to cultures containing target spores with increasing concentrations ranging from 5 × 104 to 5 × 108 spores/mL. By monitoring the shift in the resonance frequency of the MSMCs, the spores were detected in a real-time manner and a detection limit of 105 spores/mL was obtained for the MSMCs used in this research. Higher sensitivity is expected for the MSMCs with smaller size.

Keywords

sensorbiologicalmagnetic
Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 951: Symposium E – Nanofunctional Materials, Nanostructures and Novel Devices for Biological and Chemical Detection , 2006 , 0951-E05-04
Copyright
Copyright © Materials Research Society 2007

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References

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