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Control of Enzymatic Activities by Magnetite Nanoparticles

Published online by Cambridge University Press:  01 February 2011

Hui Zhou ,
Marie-Eve Aubin-Tam  and
Kimberly Hamad-Schifferli
Hui Zhou
Affiliation:
zhouhui@mit.edu, Massachusetts Institute of Technology, Materials Science and Engineering, 77 Massachusetts Avenue, Room 56-367, Cambridge, MA, 02142, United States, 617-324-0251
Marie-Eve Aubin-Tam
Affiliation:
meaubin@MIT.EDU, Massachusetts Institute of Technology, Biological Engineering Division, Cambridge, MA, 02139, United States
Kimberly Hamad-Schifferli
Affiliation:
schiffer@MIT.EDU, Massachusetts Institute of Technology, Biological Engineering Division, Cambridge, MA, 02139, United States
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Abstract

Enzymes are proteins that catalyze chemical reactions, participating in almost all processes in the cell to achieve significant reaction rates and serving a wide variety of functions inside living organisms. Here, we intended to control enzymatic activities by applying external radio frequency magnetic field (RFMF) through nanosized antenna. Ribonuclease A (RNase A), which is a relative small protein that cleave single-stranded RNA, was conjugated to magnetite nanoparticles (NP) by non-covalent interaction. The diameters of Fe3O4 nanoparticles are less than 10nm. External RFMF was applied, and the enzymatic activities of RNase A were tuned at different levels by varying the frequencies and incubation times. Comparison results by using water bath were also presented.

Keywords

biomaterialnanoscalemagnetic
Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 950: Symposium D – Biosurfaces and Biointerfaces , 2006 , 0950-D15-19
Copyright
Copyright © Materials Research Society 2007

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References

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