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Calcium Phosphate Precipitation in Liposomal Suspensions

Published online by Cambridge University Press:  21 February 2011

Edward D. Eanes
Edward D. Eanes*
Affiliation:
National Institute of Dental Research, Bone Research Branch Research Associate Program, National Institute of Standards and Technology, Gaithersburg, MD 20899
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Abstract

Artificial lipid vesicles (liposomes) provide an in vitro approach for microencapsulating calcium phosphate precipitation reactions in a manner similar to that which occurs in matrix vesicles, the initial loci for extracellular mineralization in many skeletal tissues. Apatitic precipitates readily form within the aqueous interiors of liposomes prepared from phosphatidylcholine, dicetylphosphate, and cholesterol when the liposomal membranes enclosing pH 7.4 buffered PO4 solutions are made permeable to external Ca2+ with ionophores. If the external Ca solution is rendered metastable with PO4, the apatitic precipitates rapidly expand to outside the liposomes as well. The present paper describes the basic features of liposomal mineralization and presents some specific examples on how compositional alterations in the liposomal membrane and external Ca solution can affect the progress of this mineralization.

Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 174: Symposium R – Materials Synthesis Utilizing Biological Processes , 1989 , 15
Copyright
Copyright © Materials Research Society 1990

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