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Biodegradable Cell Transplantation Devices for Tissue Regeneration

Published online by Cambridge University Press:  15 February 2011

Antonios G. Mikos ,
Heidi L. Wald ,
Georgios Sarakinos ,
Susan M. Leite  and
Robert Langer
Antonios G. Mikos
Affiliation:
Department of Chemical Engineering, Massachusetts Institute of Technology, Room E25–342, 77 Massachusetts Avenue, Cambridge, MA 02139
Heidi L. Wald
Affiliation:
Department of Chemical Engineering, Massachusetts Institute of Technology, Room E25–342, 77 Massachusetts Avenue, Cambridge, MA 02139 Current affiliation: Harvard Medical School, Boston, MA 02115
Georgios Sarakinos
Affiliation:
Department of Chemistry, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, MA 02139
Susan M. Leite
Affiliation:
Department of Chemical Engineering, Massachusetts Institute of Technology, Room E25–342, 77 Massachusetts Avenue, Cambridge, MA 02139
Robert Langer
Affiliation:
Department of Chemical Engineering, Massachusetts Institute of Technology, Room E25–342, 77 Massachusetts Avenue, Cambridge, MA 02139
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Abstract

Biodegradable polymers can be utilized as templates for cell transplantation and regeneration of metabolic organs and structural tissues. Candidate materials must be adhesive substrates for cells, promote cell growth and allow for retention of cell function. However, the processing requirements of such materials into highly porous three-dimensional structures with large surface per volume and an interconnecting pore network limits their potential application for tissue regeneration. A new processing technique was developed to produce uniform, three-dimensional cell transplantation devices of poly(lactic-co-glycolic acid). The process involved the preparation of highly porous membranes by a solvent-casting and particulate-leaching technique followed by their lamination. The device structural and mechanical properties depended on those of their constituent membranes, as evaluated by mercury porosimetry, scanning electron microscopy, and thermomechanical analysis. Cells to be seeded into the devices were injected from catheters incorporated within their structure. In vitro studies with model suspensions of dyed microspheres allowed for visual evaluation of the internal pore structure of various layered devices. From these studies, numerous parameters of device design for cell seeding were determined including pore size and injection rate. The membrane lamination technique produced devices without interfaces between layers as determined by microsphere injection and scanning electron microscopy.

Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 252: Symposium T – Tissue-Inducing Biomaterials , 1991 , 353
Copyright
Copyright © Materials Research Society 1992

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References

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