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Adsorption of DNA onto Charged Spheres

Published online by Cambridge University Press:  01 February 2011

Alison J. Hodrien ,
Alison M. Voice  and
Thomas A. Waigh
Alison J. Hodrien
Affiliation:
School of Physics and Astronomy, University of Leeds, Leeds, LS2 9JT, UK.
Alison M. Voice
Affiliation:
School of Physics and Astronomy, University of Leeds, Leeds, LS2 9JT, UK.
Thomas A. Waigh
Affiliation:
School of Physics and Astronomy, University of Leeds, Leeds, LS2 9JT, UK.
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Abstract

The complexation behaviour of linear DNA (negatively charged) with surface functionalised sub-micron latex spheres is studied by dynamic light scattering (DLS), small-angle neutron scattering (SANS), small-angle x-ray scattering (SAXS), and micro rheology. The complexes are measured in solution as a function of component concentration (CDNA, CSPH) and added salt concentration (Cs). In the absence of salt, measured radius increases with CDNA (CSPH held constant) up to a plateau value. The presence of salt causes a decrease in the measured complex radius, which may be due either to increased flexibility of the chains allowing them to form a more compact layer on the sphere surface, or dissociation of the two components due to screened electrostatics. More detailed experiments to determine this are underway.

Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 856: Symposium BB – Multicomponent Polymer Systems-Phase Behavior, Dynamics, and Applications , 2004 , BB10.1
Copyright
Copyright © Materials Research Society 2005

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References

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