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Targeting of a magnetic bionanomaterial to HepG2 human hepatocellular carcinoma cells using a galactose terminated lipid

Published online by Cambridge University Press:  03 June 2014

Andrew Booth ,
Thomas P. Coxon ,
Julie E. Gough  and
Simon J. Webb
Andrew Booth
Affiliation:
School of Chemistry and Manchester Institute of Biotechnology, University of Manchester, 131 Princess Street, Manchester, M1 7DN, United Kingdom School of Materials, University of Manchester, M13 9PL, United Kingdom
Thomas P. Coxon
Affiliation:
School of Chemistry and Manchester Institute of Biotechnology, University of Manchester, 131 Princess Street, Manchester, M1 7DN, United Kingdom
Julie E. Gough
Affiliation:
School of Materials, University of Manchester, M13 9PL, United Kingdom
Simon J. Webb
Affiliation:
School of Chemistry and Manchester Institute of Biotechnology, University of Manchester, 131 Princess Street, Manchester, M1 7DN, United Kingdom
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Abstract

Magnetic nanoparticle-vesicle aggregates (MNPVs), a controlled release nanostructure, have been enhanced with the inclusion of a novel galactose terminated lipid for cell targeting. Quartz crystal microgravimetry with dissipation (QCM-D) demonstrated that the galactose headgroup was available to bind Erythrina Crista-galli lectin (ECL) when the lipid was incorporated into a lipid bilayer. Similarly, UV-visible spectrophotometry indicated that ECL recognized the galactose headgroup in vesicles, leading to vesicle adhesion and aggregation. Finally, confocal fluorescence microscopy was used to assess the galactose-mediated interaction of both vesicles and MNPVs with HepG2 human hepatocellular carcinoma cells expressing the asialoglycoprotein (ASGPR) galactose receptor.

Keywords

chemical synthesisbiomaterialnanostructure
Type
Articles
Information
MRS Online Proceedings Library (OPL) , Volume 1688: Symposium Y – Biomaterials for Biomolecule Delivery and Understanding Cell-Niche Interactions , 2014 , mrss14-1688-y05-14
Copyright
Copyright © Materials Research Society 2014 

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