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Surface Analytical Characterization of P3HT-Streptavidin Bilayers for Biosensing Applications

Published online by Cambridge University Press:  22 June 2015

Maria Chiara Sportelli
Affiliation:
Dipartimento di Chimica, Università degli Studi Bari “Aldo Moro”, Via E. Orabona 4, 70126 Bari, Italy.
Rosaria Anna Picca
Affiliation:
Dipartimento di Chimica, Università degli Studi Bari “Aldo Moro”, Via E. Orabona 4, 70126 Bari, Italy.
Kyriaki Manoli
Affiliation:
Dipartimento di Chimica, Università degli Studi Bari “Aldo Moro”, Via E. Orabona 4, 70126 Bari, Italy.
Maria Magliulo
Affiliation:
Dipartimento di Chimica, Università degli Studi Bari “Aldo Moro”, Via E. Orabona 4, 70126 Bari, Italy.
Marilena Re
Affiliation:
ENEA - Unità Tecnica Tecnologia dei Materiali – Sez. Materiali Compositi e Nanostrutturati S.S. 7 “APPIA” KM 706, 72100 Brindisi, Italy.
Emanuela Pesce
Affiliation:
ENEA - Unità Tecnica Tecnologia dei Materiali – Sez. Materiali Compositi e Nanostrutturati S.S. 7 “APPIA” KM 706, 72100 Brindisi, Italy.
Leander Tapfer
Affiliation:
ENEA - Unità Tecnica Tecnologia dei Materiali – Sez. Materiali Compositi e Nanostrutturati S.S. 7 “APPIA” KM 706, 72100 Brindisi, Italy.
Maria Vittoria Santacroce
Affiliation:
CNR-IFN and Dipartimento Interateneo di Fisica, Università degli Studi Bari “Aldo Moro”, Via Amendola 173, 70126 Bari, Italy.
Cinzia Di Franco
Affiliation:
CNR-IFN and Dipartimento Interateneo di Fisica, Università degli Studi Bari “Aldo Moro”, Via Amendola 173, 70126 Bari, Italy.
Nicola Cioffi
Affiliation:
Dipartimento di Chimica, Università degli Studi Bari “Aldo Moro”, Via E. Orabona 4, 70126 Bari, Italy.
Luisa Torsi
Affiliation:
Dipartimento di Chimica, Università degli Studi Bari “Aldo Moro”, Via E. Orabona 4, 70126 Bari, Italy.
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Abstract

Ultrasensitive biosensors based on bottom gate organic field-effect transistors can be developed by depositing a functional biological (protein) interlayer directly on the silicon oxide gate dielectric and underneath the organic semiconductor film. However, the deposition methods for assembling the protein biological recognition layer can affect the biosensor analytical performances for the target analyte detection. Here, spin-coating and layer-by-layer techniques were considered as different approaches for streptavidin protein deposition. X-ray photoelectron spectroscopy (XPS) was systematically used in the non-destructive parallel angle resolved mode to characterize the multilayer device at each step of its assembly to gain information on elemental depth profiles. Scanning electron and scanning Helium ion microscopies gave information about stacked layer structure and morphology corroborating XPS results.

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Articles
Copyright
Copyright © Materials Research Society 2015 

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References

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