Skip to main content Accessibility help
×
Home
Hostname: page-component-cf9d5c678-dksz7 Total loading time: 0.202 Render date: 2021-07-29T07:55:40.000Z Has data issue: true Feature Flags: { "shouldUseShareProductTool": true, "shouldUseHypothesis": true, "isUnsiloEnabled": true, "metricsAbstractViews": false, "figures": true, "newCiteModal": false, "newCitedByModal": true, "newEcommerce": true, "newUsageEvents": true }

Surface Analytical Characterization of P3HT-Streptavidin Bilayers for Biosensing Applications

Published online by Cambridge University Press:  22 June 2015

Maria Chiara Sportelli
Affiliation:
Dipartimento di Chimica, Università degli Studi Bari “Aldo Moro”, Via E. Orabona 4, 70126 Bari, Italy.
Rosaria Anna Picca
Affiliation:
Dipartimento di Chimica, Università degli Studi Bari “Aldo Moro”, Via E. Orabona 4, 70126 Bari, Italy.
Kyriaki Manoli
Affiliation:
Dipartimento di Chimica, Università degli Studi Bari “Aldo Moro”, Via E. Orabona 4, 70126 Bari, Italy.
Maria Magliulo
Affiliation:
Dipartimento di Chimica, Università degli Studi Bari “Aldo Moro”, Via E. Orabona 4, 70126 Bari, Italy.
Marilena Re
Affiliation:
ENEA - Unità Tecnica Tecnologia dei Materiali – Sez. Materiali Compositi e Nanostrutturati S.S. 7 “APPIA” KM 706, 72100 Brindisi, Italy.
Emanuela Pesce
Affiliation:
ENEA - Unità Tecnica Tecnologia dei Materiali – Sez. Materiali Compositi e Nanostrutturati S.S. 7 “APPIA” KM 706, 72100 Brindisi, Italy.
Leander Tapfer
Affiliation:
ENEA - Unità Tecnica Tecnologia dei Materiali – Sez. Materiali Compositi e Nanostrutturati S.S. 7 “APPIA” KM 706, 72100 Brindisi, Italy.
Maria Vittoria Santacroce
Affiliation:
CNR-IFN and Dipartimento Interateneo di Fisica, Università degli Studi Bari “Aldo Moro”, Via Amendola 173, 70126 Bari, Italy.
Cinzia Di Franco
Affiliation:
CNR-IFN and Dipartimento Interateneo di Fisica, Università degli Studi Bari “Aldo Moro”, Via Amendola 173, 70126 Bari, Italy.
Nicola Cioffi
Affiliation:
Dipartimento di Chimica, Università degli Studi Bari “Aldo Moro”, Via E. Orabona 4, 70126 Bari, Italy.
Luisa Torsi
Affiliation:
Dipartimento di Chimica, Università degli Studi Bari “Aldo Moro”, Via E. Orabona 4, 70126 Bari, Italy.
Get access

Abstract

Ultrasensitive biosensors based on bottom gate organic field-effect transistors can be developed by depositing a functional biological (protein) interlayer directly on the silicon oxide gate dielectric and underneath the organic semiconductor film. However, the deposition methods for assembling the protein biological recognition layer can affect the biosensor analytical performances for the target analyte detection. Here, spin-coating and layer-by-layer techniques were considered as different approaches for streptavidin protein deposition. X-ray photoelectron spectroscopy (XPS) was systematically used in the non-destructive parallel angle resolved mode to characterize the multilayer device at each step of its assembly to gain information on elemental depth profiles. Scanning electron and scanning Helium ion microscopies gave information about stacked layer structure and morphology corroborating XPS results.

Type
Articles
Copyright
Copyright © Materials Research Society 2015 

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Torsi, L., Magliulo, M., Manoli, K., and Palazzo, G., Chem. Soc. Rev. 42, 8612 (2013).CrossRef
Angione, M. D., Cotrone, S., Magliulo, M., Mallardi, A., Altamura, D., Giannini, C., Cioffi, N., Sabbatini, L., Fratini, E., Baglioni, P., Scamarcio, G., Palazzo, G., and Torsi, L., Proc. Natl. Acad. Sci. 109, 6429 (2012).CrossRef
Magliulo, M., Mallardi, A., Gristina, R., Ridi, F., Sabbatini, L., Cioffi, N., Palazzo, G., and Torsi, L., Anal. Chem. 85, 3849 (2013).CrossRef
Pilolli, R., Ditaranto, N., Cioffi, N., and Sabbatini, L., Anal. Bioanal. Chem. 405, 713 (2013).CrossRef
Cho, S., Lee, K., Yuen, J., Wang, G., Moses, D., Heeger, A. J., Surin, M., and Lazzaroni, R., J. Appl. Phys. 100, 114503 (2006).CrossRef
Fang, M., Tang, Z., Lu, H., and Nutt, S., J. Mater. Chem. 22, 109 (2012).CrossRef
Magliulo, M., Altamura, D., Di Franco, C., Santacroce, M. V., Manoli, K., Mallardi, A., Palazzo, G., Scamarcio, G., Giannini, C., and Torsi, L., J. Phys. Chem. C 118, 15853 (2014).CrossRef
Werner, C., Eichhorn, K.-J., Grundke, K., Simon, F., Grählert, W., and Jacobasch, H.-J., Colloids Surf. Physicochem. Eng. Asp. 156, 3 (1999).CrossRef
Lvov, Y., Ariga, K., Ichinose, I., and Kunitake, T., J. Am. Chem. Soc. 117, 6117 (1995).CrossRef

Send article to Kindle

To send this article to your Kindle, first ensure no-reply@cambridge.org is added to your Approved Personal Document E-mail List under your Personal Document Settings on the Manage Your Content and Devices page of your Amazon account. Then enter the ‘name’ part of your Kindle email address below. Find out more about sending to your Kindle. Find out more about sending to your Kindle.

Note you can select to send to either the @free.kindle.com or @kindle.com variations. ‘@free.kindle.com’ emails are free but can only be sent to your device when it is connected to wi-fi. ‘@kindle.com’ emails can be delivered even when you are not connected to wi-fi, but note that service fees apply.

Find out more about the Kindle Personal Document Service.

Surface Analytical Characterization of P3HT-Streptavidin Bilayers for Biosensing Applications
Available formats
×

Send article to Dropbox

To send this article to your Dropbox account, please select one or more formats and confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your <service> account. Find out more about sending content to Dropbox.

Surface Analytical Characterization of P3HT-Streptavidin Bilayers for Biosensing Applications
Available formats
×

Send article to Google Drive

To send this article to your Google Drive account, please select one or more formats and confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your <service> account. Find out more about sending content to Google Drive.

Surface Analytical Characterization of P3HT-Streptavidin Bilayers for Biosensing Applications
Available formats
×
×

Reply to: Submit a response

Please enter your response.

Your details

Please enter a valid email address.

Conflicting interests

Do you have any conflicting interests? *