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Identifying Iron Oxide Based Materials that Can Either Pass or Not Pass through the in vitro Blood-Brain Barrier

Published online by Cambridge University Press:  17 February 2014

Di Shi
Affiliation:
Department of Pharmaceutical Sciences, Northeastern University, Boston, MA 02115, USA.
Linlin Sun
Affiliation:
Department of Bioengineering, Northeastern University, Boston, MA 02115, USA.
Gujie Mi
Affiliation:
Department of Pharmaceutical Sciences, Northeastern University, Boston, MA 02115, USA.
Soumya Bhattacharya
Affiliation:
Materials Science and Technology Division, CSIR-National Metallurgical Laboratory, Jamshedpur, JH 831007, India.
Suprabha Nayar
Affiliation:
Materials Science and Technology Division, CSIR-National Metallurgical Laboratory, Jamshedpur, JH 831007, India.
Thomas J Webster
Affiliation:
Department of Chemical Engineering, Northeastern University, Boston, MA 02115, USA.
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Abstract

In this study, an in vitro blood-brain barrier model was developed using murine brain endothelioma cells (b.End3 cells). By comparing the permeability of FITC-Dextran at increasing exposure times in serum-free medium to such values in the literature, we confirm that the blood-brain barrier model was successfully established. After such confirmation, the permeability of five ferrofluid (FF) nanoparticle samples, GGB (ferrofluid synthesized using glycine, glutamic acid and BSA), GGC (glycine, glutamic acid and collagen), GGP (glycine, glutamic acid and PVA), BPC (BSA, PEG and collagen) and CPB (collagen, PVA and BSA), was determined using this model. In addition, all the five FF samples were characterized by zeta potential to determine their charge as well as TEM and dynamic light scattering for determining their hydrodynamic diameter. Results showed that FF coated with collagen had better permeability to the blood-brain barrier than FF coated with glycine and glutamic acid based on an increase of 4.5% in permeability. Through such experiments, magnetic nanomaterials, such as ferrofluids, that are less permeable to the blood brain barrier can be used to decrease neural tissue toxicity and magnetic nanomaterials with more permeable to the blood-brain barrier can be used for brain drug delivery.

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Articles
Copyright
Copyright © Materials Research Society 2014 

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References

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