Skip to main content Accessibility help
×
Home
Hostname: page-component-564cf476b6-scc96 Total loading time: 0.372 Render date: 2021-06-19T08:03:56.486Z Has data issue: true Feature Flags: { "shouldUseShareProductTool": true, "shouldUseHypothesis": true, "isUnsiloEnabled": true, "metricsAbstractViews": false, "figures": true, "newCiteModal": false, "newCitedByModal": true, "newEcommerce": true }

Gold Nanoparticle Enlargement Coupled with Fluorescence Decrease for Highly Sensitive Detection of Analytes

Published online by Cambridge University Press:  28 January 2011

Seong Yoon Lim
Affiliation:
Department of Materials Science and Engineering, Korea Advanced Institute of Science and Technology 335 Science Road, Daejeon 305-701, Republic of Korea
Jae Hong Kim
Affiliation:
Department of Materials Science and Engineering, Korea Advanced Institute of Science and Technology 335 Science Road, Daejeon 305-701, Republic of Korea
Joon Seok Lee
Affiliation:
Department of Materials Science and Engineering, Korea Advanced Institute of Science and Technology 335 Science Road, Daejeon 305-701, Republic of Korea
Chan Beum Park
Affiliation:
Department of Materials Science and Engineering, Korea Advanced Institute of Science and Technology 335 Science Road, Daejeon 305-701, Republic of Korea
Get access

Abstract

We present a versatile and facile route for highly sensitive detection of analytes through coupling the enlargement of gold nanoparticles (Au NPs) with fluorescence decrease. The fluorescence intensity of dye molecules (e.g., fluorescein or rhodamine B) significantly decreased with the increasing concentration of reducing agents, such as hydrogen peroxide and hydroquinone. The sensitivity for the detection of reducing agents was much higher than other detection methods based on the absorbance measurement of enlarged gold nanoparticles or quantum dot-enzyme hybridization. We could successfully detect acetylthiocholine with the detection limit of several nM orders, using an enzymatic reaction by acetylcholinesterase, a key route for the detection of toxic organophosphate compounds. The fluorescence decreasing approach described in this work requires only a simple addition of fluorescence dye to the reaction solution without any chemical modification. The strategy of fluorescence decrease coupled with nanoparticle growth will be applied on the fluorescent substrate to develop detection templates for highly sensitive optical biosensor.

Type
Research Article
Copyright
Copyright © Materials Research Society 2011

Access options

Get access to the full version of this content by using one of the access options below.

References

1. Zayats, M.; Baron, R.; Popov, I.; Willner, I. Nano Lett. 2005, 5, 2125.10.1021/nl048547pCrossRefGoogle Scholar
2. Jagetia, G. C.; Menon, K. S. L.; Jain, V. Toxicol. Lett. 2001, 121, 1520.10.1016/S0378-4274(00)00290-3CrossRefGoogle Scholar
3. Dutta, K.; Bhattacharyay, D.; Mukherjee, A.; Setford, S. J.; Turner, A. P. F.; Sarkar, P. Ecotox. Environ. Safe. 2008, 69, 556561.10.1016/j.ecoenv.2007.01.004CrossRefGoogle Scholar
4. Bültzingslöwen, C.; McEvoy, A. K.; McDonagh, C.; MacCraith, B. D. Anal. Chim. Acta 2003, 480, 275283.10.1016/S0003-2670(02)01653-7CrossRefGoogle Scholar
5. Pickup, J. C.; Hussain, F.; Evans, N. D.; Rolinski, O. J.; Birch, D. J. S. Biosens. Bioelectron. 2005, 20, 25552565.10.1016/j.bios.2004.10.002CrossRefGoogle Scholar
6. Griffin, J.; Singh, A. K.; Senapati, D.; Rhodes, P.; Mitchell, K.; Robinson, B.; Yu, E.; Ray, P. C. Chem. Eur. J. 2009, 15, 342351.10.1002/chem.200801812CrossRefGoogle Scholar
7. Lee, S.; Cha, E.-J.; Park, K.; Lee, S.-Y.; Hong, J.-K.; Sun, I.-C.; Kim, S. Y.; Choi, K.; Kwon, I. C.; Kim, K. et al. Angew. Chem. Int. Ed. 2008, 47, 28042807.10.1002/anie.200705240CrossRefGoogle Scholar
8. Maxwell, D. J.; Taylor, J. R.; Nie, S. J. Am. Chem. Soc. 2002, 124, 96069612.10.1021/ja025814pCrossRefGoogle Scholar
9. Zhu, L.; Lee, C. S.; DeVoe. D. L. Lab Chip 2006, 6, 115120.10.1039/B511766FCrossRefGoogle Scholar
10. Yuan, J.; Guo, W.; Wang, E. Anal. Chem. 2008, 80, 11411145.10.1021/ac0713048CrossRefGoogle Scholar

Send article to Kindle

To send this article to your Kindle, first ensure no-reply@cambridge.org is added to your Approved Personal Document E-mail List under your Personal Document Settings on the Manage Your Content and Devices page of your Amazon account. Then enter the ‘name’ part of your Kindle email address below. Find out more about sending to your Kindle. Find out more about sending to your Kindle.

Note you can select to send to either the @free.kindle.com or @kindle.com variations. ‘@free.kindle.com’ emails are free but can only be sent to your device when it is connected to wi-fi. ‘@kindle.com’ emails can be delivered even when you are not connected to wi-fi, but note that service fees apply.

Find out more about the Kindle Personal Document Service.

Gold Nanoparticle Enlargement Coupled with Fluorescence Decrease for Highly Sensitive Detection of Analytes
Available formats
×

Send article to Dropbox

To send this article to your Dropbox account, please select one or more formats and confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your <service> account. Find out more about sending content to Dropbox.

Gold Nanoparticle Enlargement Coupled with Fluorescence Decrease for Highly Sensitive Detection of Analytes
Available formats
×

Send article to Google Drive

To send this article to your Google Drive account, please select one or more formats and confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your <service> account. Find out more about sending content to Google Drive.

Gold Nanoparticle Enlargement Coupled with Fluorescence Decrease for Highly Sensitive Detection of Analytes
Available formats
×
×

Reply to: Submit a response

Please enter your response.

Your details

Please enter a valid email address.

Conflicting interests

Do you have any conflicting interests? *