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Functionalized Magnetic Nanoparticles for Selective Targeting of Cells

Published online by Cambridge University Press:  31 January 2011

Wolfgang Tremel
Affiliation:
shukoor@uni-mainz.de, Universität Mainz, Institut für Anorganische Chemie und Analytische Chemie, Mainz, Germany
Mohammed Ibrahim Shukoor
Affiliation:
natalio@uni-mainz.de, Universität Mainz, Institut für Physiologische Chemie, Mainz, Germany
Filipe Natalio
Affiliation:
schladt@uni-mainz.de, Germany
Thomas Schladt
Affiliation:
barz@uni-mainz.de, Johannes Gutenberg-Universität Mainz, Institut für Organische Chemie, Mainz, Germany
Matthias Barz
Affiliation:
tahir@uni-mainz.de, Germany
Muhammad Nawaz Tahir
Affiliation:
stefanw@uni-mainz.de, Klinikum der Johannes Gutenberg-Universität Mainz, Klinik und Poliklinik für Diagnostische und Interventionelle Radiologie, Mainz, Germany
Stefan Weber
Affiliation:
zentel@uni-mainz.de, Johannes Gutenberg-Universität Mainz, Institut für Organische Chemie, Mainz, Germany
Rudolf Zentel
Affiliation:
lschreib@uni-mainz.de, Klinikum der Johannes Gutenberg-Universität Mainz, Klinik und Poliklinik für Diagnostische und Interventionelle Radiologie, Mainz, Germany
Laura Maria Schreiber
Affiliation:
brieger@hno.klinik.uni-mainz.de, Klinikum der Johannes Gutenberg-Universität Mainz, HNO Klinik, Labor für Tumorbiologie, Mainz, Germany
Jürgen Brieger
Affiliation:
hschroed@uni-mainz.de, Germany
Heinz Christoph Schröder
Affiliation:
wmueller@uni-mainz.de, Germany
Werner Müller
Affiliation:
tremel@uni-mainz.de, Universität Mainz, Institut für Anorganische Chemie und Analytische Chemie, Duesbergweg 10-14, Mainz, 55099, Germany, +49 6131 3925135, +49 6131 3925605
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Abstract

Initiation of pathways that lead to proliferation and chemoresistance by Toll-like receptors (TLRs) is an important factor in cancer progression. Here, we show the response of human cancer cells to TLR signaling inevitably linked to tumor biology. The approach is based on tailored multifunctional magnetic nanoparticles equipped with pathogen-derived ligands (CpG) functioning as TLR agonists (molecular component) to investigate the impact of transcription factor immune activation on human cancer cells. Magnetic nanoparticles (MnO and bifunctional Au-MnO) particles were covalently coated with a multifunctional polymer, displaying no cytotoxicity, to being able to enter cells while carrying foreign DNA (unmethylated CpG) to recognize intracellular TLR 9. Both, the particle and the nucleic acid are tagged with fluorescent markers for simultaneous visualization inside the cell. Apart from optical imaging, the magnetism of the particles also allows magnetic resonance imaging of organisms.

Type
Research Article
Copyright
Copyright © Materials Research Society 2010

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