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Electrochemical Release of Immobilized IgG Protein

Published online by Cambridge University Press:  01 February 2011

Tanveer Mahmud
Affiliation:
e65209@rmit.edu.au, RMIT University, School of Electrical and Computer Engineering, Melbourne, 3001, Australia
Wojtek Wlodarski
Affiliation:
ww@rmit.edu.au, RMIT University, School of Electrical and Computer Engineering, Melbourne, 3001, Australia
Arnan Mitchell
Affiliation:
arnan.mitchell@rmit.edu.au, RMIT University, School of Electrical and Computer Engineering, Melbourne, 3001, Australia
Sally Gras
Affiliation:
sgras@unimelb.edu.au, The University of Melbourne, Department of Chemical and Biomolecular Engineering, and The Bio21 Molecular Science & Biotechnology, Melbourne, 3004, Australia
Adrian Trinchi
Affiliation:
adrian.trinchi@csiro.au, CSIRO, Manufacturing and Materials Technology, Melbourne, N/A, Australia
Kourosh Kalantar-zadeh
Affiliation:
kourosh.kalantar@rmit.edu.au, RMIT University, School of Electrical and Computer Engineering, Melbourne, 3001, Australia
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Abstract

In this paper, we present the electrochemically programmed release of immobilized IgG protein molecules that have been attached to gold coated surfaces via a thiol-gold linkage. Fluorescence microscopy has been used to image the release of fluorescently tagged IgGs in phosphate buffered saline. In this technique, the reductive desorption of self-assembled monolayers is employed for the release of proteins, which are immobilized on the surface either by non-covalent or covalent interactions. The voltage applied for the release of proteins is in a range of -1.5V to -60V.

Type
Research Article
Copyright
Copyright © Materials Research Society 2007

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References

1. Nuzzo, R.G. and Allara, D.L., “Adsorption of bifunctional organic disulfides on gold surfaces”, Journal of American Chemical Society, vol. 105, pp. 44814483, 1983.CrossRefGoogle Scholar
2. Porter, M.D., Bright, T.B., Allara, D.L., Chidseyi, C.E.D., “Spontaneously organized molecular assemblies .4. structural characterization of n-alkyl thiol monolayers on gold by optical ellipsometry, infrared spectroscopy, and electrochemistry”, Journal of American Chemical Society, vol. 109, pp. 35593568, 1987.CrossRefGoogle Scholar
3. Mali, P., Bhattacharjee, N., and Searson, P.C., “Electrochemically programmed release of biomolecules and nanoparticles”, Nano Letters, vol. 6, pp. 12501253, 2006.CrossRefGoogle ScholarPubMed

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