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Electrochemical detection of non-labeling DNA using electronic array

Published online by Cambridge University Press:  11 February 2011

H. Y. Lee
Affiliation:
The Institute of Scientific and Industrial Research, Osaka University, 8–1 Mihogaoka, Ibaraki, Osaka 567–0047, Japan
J. W. Park
Affiliation:
The Institute of Scientific and Industrial Research, Osaka University, 8–1 Mihogaoka, Ibaraki, Osaka 567–0047, Japan
Y. S. Choi
Affiliation:
The Institute of Scientific and Industrial Research, Osaka University, 8–1 Mihogaoka, Ibaraki, Osaka 567–0047, Japan
T. Kannno
Affiliation:
The Institute of Scientific and Industrial Research, Osaka University, 8–1 Mihogaoka, Ibaraki, Osaka 567–0047, Japan
Hiro. Tanaka
Affiliation:
The Institute of Scientific and Industrial Research, Osaka University, 8–1 Mihogaoka, Ibaraki, Osaka 567–0047, Japan
Hide. Tanaka
Affiliation:
The Institute of Scientific and Industrial Research, Osaka University, 8–1 Mihogaoka, Ibaraki, Osaka 567–0047, Japan
T. Kawai*
Affiliation:
The Institute of Scientific and Industrial Research, Osaka University, 8–1 Mihogaoka, Ibaraki, Osaka 567–0047, Japan
*
a) Electronic mail: kawai@sanken.osaka-u.ac.jp
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Abstract

Our system is the electrochemical approaches include the detection of hybridization from nonlabeling nucleic acids to protein-bound nucleic acids using soluble mediators with K4Fe(CN)6 solutions. In order to generate bio-functional surfaces, the streptavidin(SAv)-biotin system is used. A 50 % change of redox peak current after hybridization measured with 50 ?M concentration of target DNA. We suggest that this result comes from the efficient electron transport through the SAv-biotin interaction. Our electrochemical detection system showed good reproducibility on a chip with non-labeling DNA hybridization detection.

Type
Research Article
Copyright
Copyright © Materials Research Society 2003

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References

1. Kawai, T., Proc. μTAS2002, 1, 43 (2002)Google Scholar
2. Tiefenauer, Louis, et al. Biosensor & Biochemistry, 15, 431(2000)Google Scholar
3. Sosnowski, Ronald G., et al. Proc. Natl. Acad. Sci., 94, 1119 (1997)CrossRefGoogle Scholar

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