Skip to main content Accessibility help
×
Home
Hostname: page-component-568f69f84b-tcbk7 Total loading time: 0.177 Render date: 2021-09-18T04:01:07.408Z Has data issue: true Feature Flags: { "shouldUseShareProductTool": true, "shouldUseHypothesis": true, "isUnsiloEnabled": true, "metricsAbstractViews": false, "figures": true, "newCiteModal": false, "newCitedByModal": true, "newEcommerce": true, "newUsageEvents": true }

Biofunctional Thermo-Responsive Polymeric Surface with Micropatterns for Label Free Cell Separation

Published online by Cambridge University Press:  17 February 2014

Yoshikazu Kumashiro
Affiliation:
Institute of Advanced Biomedical Engineering and Science, Tokyo Women’s Medical University, 8-1 Kawada-cho, Shinjuku-ku,Tokyo 162-8666, Japan
Jun Ishihara
Affiliation:
Institute of Advanced Biomedical Engineering and Science, Tokyo Women’s Medical University, 8-1 Kawada-cho, Shinjuku-ku,Tokyo 162-8666, Japan Division of Cellular Therapy, The Institute of Medical Science, University of Tokyo, 4-6-1 Shirokanedai, Minato-ku, Tokyo 108-8039, Japan.
Terumasa Umemoto
Affiliation:
Institute of Advanced Biomedical Engineering and Science, Tokyo Women’s Medical University, 8-1 Kawada-cho, Shinjuku-ku,Tokyo 162-8666, Japan
Kazuyoshi Itoga
Affiliation:
Institute of Advanced Biomedical Engineering and Science, Tokyo Women’s Medical University, 8-1 Kawada-cho, Shinjuku-ku,Tokyo 162-8666, Japan
Jun Kobayashi
Affiliation:
Institute of Advanced Biomedical Engineering and Science, Tokyo Women’s Medical University, 8-1 Kawada-cho, Shinjuku-ku,Tokyo 162-8666, Japan
Masayuki Yamato
Affiliation:
Institute of Advanced Biomedical Engineering and Science, Tokyo Women’s Medical University, 8-1 Kawada-cho, Shinjuku-ku,Tokyo 162-8666, Japan
Teruo Okano
Affiliation:
Institute of Advanced Biomedical Engineering and Science, Tokyo Women’s Medical University, 8-1 Kawada-cho, Shinjuku-ku,Tokyo 162-8666, Japan
Get access

Abstract

Thready stripe-patterned thermo-responsive surfaces were prepared and their surface properties were characterized. Prepared 3 μm wide stripe-patterned surfaces were evaluated by observing the adhesions and detachments of three types of cells: HeLa cells (HeLas), human umbilical vein endothelial cells (HUVECs), and NIH-3T3 cells (3T3s). Although cell adhesion and detachment in response to temperature were observed on all cells on a conventional thermo-responsive surface without patterns, the thermo-responsive surface with a 3 μm striped-pattern exhibited various cell adhesion properties. HeLas hardly adhered to the patterned surface even at 37 °C. On the other hand, although HUVECs adhered on the patterned surface at 12 h after incubation at 37 °C, the adhered HUVECs detached themselves after another 12 h incubation at 37 °C. 3T3s adhered to the patterned surface at 37 °C and detached themselves after reducing temperature to 20 °C. A mixture of HeLa, HUVEC and 3T3 was separated using their different specific cell-adhesion properties, and the composition of cells was analyzed by a flow-cytometry. As a result, the conventional thermo-responsive surface with a stripe-pattern was found to function as a cell-separating interface by using specific cell adhesion properties.

Type
Articles
Copyright
Copyright © Materials Research Society 2014 

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Gu, Z.-Z., Uetsuka, H., Takahashi, K., Nakajima, R., Onishi, H., Fujishima, A., and Sato, O., Angew. Chem. Int. Ed. 42, 894 (2003).CrossRef
Patankar, N. A., Langmuir 20, 8209 (2004).CrossRef
Yu, X., Wang, Z., Jiang, Y., Shi, F., and Zhang, X., Adv. Mater. 17, 1289 (2005).CrossRef
Tauk, L., Schröder, A. P., Decher, G., and Giuseppone, N., Nat Chem 1, 649 (2009).CrossRef
Chen, L., Liu, M., Bai, H., Chen, P., Xia, F., Han, D., and Jiang, L., J. Am. Chem. Soc. 131, 10467 (2009).CrossRef
Jiang, Y., Wan, P., Smet, M., Wang, Z., and Zhang, X., Adv. Mater. 20, 1972 (2008).CrossRef
Ge, H., Wang, G., He, Y., Wang, X., Song, Y., Jiang, L., and Zhu, D., ChemPhysChem 7, 575 (2006).CrossRef
Masuda, T., Hidaka, M., Murase, Y., Akimoto, A. M., Nagase, K., Okano, T., and Yoshida, R., Angew. Chem. Int. Ed. 52, 7468 (2013).CrossRef
Diebold, U., Surf. Sci. Rep. 48, 53 (2003).CrossRef
Yamato, M., and Okano, T., Mater. Today 7, 42 (2004).CrossRef
Kikuchi, A., and Okano, T., J. Controlled Release 101, 69 (2005).CrossRef
Kumashiro, Y., Yamato, M., and Okano, T., Ann. Biomed. Eng. 38, 1977 (2010).CrossRef
Akiyama, Y., Kikuchi, A., Yamato, M., and Okano, T., Langmuir 20, 5506 (2004).CrossRef
Nishida, K., Yamato, M., Hayashida, Y., Watanabe, K., Yamamoto, K., Adachi, E., Nagai, S., Kikuchi, A., Maeda, N., Watanabe, H., Okano, T., and Tano, Y., N. Engl. J. Med. 351, 1187 (2004).CrossRef
Kumashiro, Y., Itoga, K., Kinoshita, Y., Yamato, M., and Okano, T., Chem. Lett. 42, 741 (2013).CrossRef
Kumashiro, Y., Matsunaga, T., Muraoka, M., Tanaka, N., Itoga, K., Kobayashi, J., Tomiyama, Y., Kuroda, M., Shimizu, T., Hashimoto, I., Umemura, K., Yamato, M., and Okano, T., J. Biomed. Mater. Res. A, (2013) in press.

Send article to Kindle

To send this article to your Kindle, first ensure no-reply@cambridge.org is added to your Approved Personal Document E-mail List under your Personal Document Settings on the Manage Your Content and Devices page of your Amazon account. Then enter the ‘name’ part of your Kindle email address below. Find out more about sending to your Kindle. Find out more about sending to your Kindle.

Note you can select to send to either the @free.kindle.com or @kindle.com variations. ‘@free.kindle.com’ emails are free but can only be sent to your device when it is connected to wi-fi. ‘@kindle.com’ emails can be delivered even when you are not connected to wi-fi, but note that service fees apply.

Find out more about the Kindle Personal Document Service.

Biofunctional Thermo-Responsive Polymeric Surface with Micropatterns for Label Free Cell Separation
Available formats
×

Send article to Dropbox

To send this article to your Dropbox account, please select one or more formats and confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your <service> account. Find out more about sending content to Dropbox.

Biofunctional Thermo-Responsive Polymeric Surface with Micropatterns for Label Free Cell Separation
Available formats
×

Send article to Google Drive

To send this article to your Google Drive account, please select one or more formats and confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your <service> account. Find out more about sending content to Google Drive.

Biofunctional Thermo-Responsive Polymeric Surface with Micropatterns for Label Free Cell Separation
Available formats
×
×

Reply to: Submit a response

Please enter your response.

Your details

Please enter a valid email address.

Conflicting interests

Do you have any conflicting interests? *