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Loading and release of a model high-molecular-weight protein from temperature-sensitive micro-structured hydrogels

Published online by Cambridge University Press:  28 June 2019

Kenia Palomino Open the ORCID record for Kenia Palomino [Opens in a new window] ,
Héctor Magaña ,
Samuel G. Meléndez-López ,
José M. Cornejo-Bravo Open the ORCID record for José M. Cornejo-Bravo [Opens in a new window] ,
Aracely Serrano-Medina ,
Manuel Alatorre-Meda ,
Eustolia Rodríguez-Velázquez  and
Ignacio Rivero
Kenia Palomino
Affiliation:
Facultad de Ciencias Químicas e Ingeniería, Universidad Autónoma de Baja California, Calzada Universidad 14418, Parque industrial Internacional, C.P. 22427 Tijuana B.C., Mexico
Héctor Magaña
Affiliation:
Facultad de Ciencias Químicas e Ingeniería, Universidad Autónoma de Baja California, Calzada Universidad 14418, Parque industrial Internacional, C.P. 22427 Tijuana B.C., Mexico
Samuel G. Meléndez-López
Affiliation:
Facultad de Ciencias Químicas e Ingeniería, Universidad Autónoma de Baja California, Calzada Universidad 14418, Parque industrial Internacional, C.P. 22427 Tijuana B.C., Mexico
José M. Cornejo-Bravo*
Affiliation:
Facultad de Ciencias Químicas e Ingeniería, Universidad Autónoma de Baja California, Calzada Universidad 14418, Parque industrial Internacional, C.P. 22427 Tijuana B.C., Mexico
Aracely Serrano-Medina
Affiliation:
Facultad de Medicina y Psicología, Universidad Autónoma de Baja California, Calzada Universidad 14418, Parque industrial Internacional, C.P. 22427 Tijuana B.C., Mexico
Manuel Alatorre-Meda
Affiliation:
Cátedras CONACyT-Tecnológico Nacional de México/I. T. Tijuana, Centro de Graduados e Investigación en Química-Grupo de Biomateriales y Nanomedicina, Blvd. Alberto Limón Padilla S/N, 22510 Tijuana B.C., Mexico
Eustolia Rodríguez-Velázquez
Affiliation:
Facultad de Odontología Campus Tijuana, Universidad Autónoma de Baja California, Calzada Universidad 14418, Parque industrial Internacional, 22390 Tijuana B.C., Mexico Tecnológico Nacional de México/I. T. Tijuana, Centro de Graduados e Investigación en Química − Grupo de Biomateriales y Nanomedicina, Blvd. Alberto Limón Padilla S/N, 22510 Tijuana B.C., Mexico
Ignacio Rivero
Affiliation:
Tecnológico Nacional de México/I. T. Tijuana, Centro de Graduados e Investigación en Química, Blvd. Alberto Limón Padilla S/N, 22510 Tijuana B.C., Mexico
*
Address all correspondence to J.M. Cornejo-Bravo at jmcornejo@uabc.edu.mx
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Abstract

The authors prepared a micro-structured, thermosensitive hydrogel with N-isopropylacrylamide microgels with a lower critical solution temperature (LCST) of 32 °C dispersed on a matrix of N-isopropylacrylamide-co-dimethylacrylamide with an LCST at 40 °C. Incubation of the hydrogel at 33 °C in a solution of fluorescein-albumin induced loading of the protein. The protein was not loaded at a temperature below the LCST of the microgels (4 °C), suggesting that the shrinkage of the microgels followed by the formation of micropores within the hydrogel matrix is a prerequisite for protein loading. A sustained and complete release of the loaded protein was obtained at 37 °C.

Type
Research Letters
Information
MRS Communications , Volume 9 , Issue 3 , September 2019 , pp. 1041 - 1045
Copyright
Copyright © Materials Research Society 2019 

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