Hostname: page-component-77c89778f8-7drxs Total loading time: 0 Render date: 2024-07-22T02:09:40.883Z Has data issue: false hasContentIssue false

Influence of Illumination Conditions on Temperature in Sample Cell and the Output of a Quadrant Detector in an Optical Tweezers System

Published online by Cambridge University Press:  14 March 2018

Yuqiang Jiang*
Affiliation:
College of Physics and Electronics Engineering, Shanxi University, TaiyuanP. R. China Institute of Physics and Center for Condensed Matter Physics, Chinese Academy of Science, BeijingP. R. China Shanxi Datong University, DatongP. R. China
Honglian Guo
Affiliation:
Institute of Physics and Center for Condensed Matter Physics, Chinese Academy of Science, BeijingP. R. China
Chunxiang Liu
Affiliation:
Institute of Physics and Center for Condensed Matter Physics, Chinese Academy of Science, BeijingP. R. China
Zhaolin Li
Affiliation:
Institute of Physics and Center for Condensed Matter Physics, Chinese Academy of Science, BeijingP. R. China
Bingying Cheng
Affiliation:
Institute of Physics and Center for Condensed Matter Physics, Chinese Academy of Science, BeijingP. R. China
Daozhong Zhang
Affiliation:
Institute of Physics and Center for Condensed Matter Physics, Chinese Academy of Science, BeijingP. R. China
Suotang Jia
Affiliation:
College of Physics and Electronics Engineering, Shanxi University, TaiyuanP. R. China

Extract

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

In an optical tweezers system, the output signal of a photodiode quadrant detector and the temperature in a sample cell are two key factors for the quantitative measurements of mechanical properties of living biological objects such as cells, organelles and macro-molecules. In order to enhance the output of a quadrant detector and effectively control the temperature in a sample cell, the dependence of the temperature in the sample cell and the output of the quadrant detector for different illumination conditions are studied. The results show that appropriate illumination conditions can ensure both nearly constant temperatures in the cell and the desired output signal, which provides for the possibility of highprecision and damage-free analysis of living biological objects.

Type
Research Article
Copyright
Copyright © Microscopy Society of America 2005

References

1. Mehta, A. D., Reif, M., Spudich, J. A., Smith, D. A. and Simmons, R. M., “Single- Molecule Biomechanics with Optical Methods,” Science 283, 1689-1695 (1999).CrossRefGoogle Scholar
2. Finer, J. T., Simmons, R. M., and Spudich, J. A., “Single Myosin Molecule Mechanics: Piconewton Forces and Nanometer Steps,” Nature 368, 113119 (1994).Google Scholar
3. LeCates, W.W., Kuo, S. C. and Brownell, W. E. Association for Research in Otolaryngology Midwinter Meeting. Abstract 622 (1995).Google Scholar
4. Lui, Y., Cheng, D. K., Soneck, G. J., Berns, M. W., Chapman, C. F., and Tromberg, B. J., “Evidence for Localized Cell Heating Induced by Infrared Optical Tweezers,” Biophys. J. 68, 21372144 (1995).Google Scholar
5. Lui, Y., Cheng, D. K., Soneck, G. J., Berns, M. W. and Tromberg, B. J., “Microfluorometric Technique for the Determination of Localized Heating in Organic Particles,” Appl. Phys. Lett. 65 919921 (1994).Google Scholar
6. Mietchen, D., Schnelle, T., Müller, T., Hagedorn, and Fuhr, G. J., “Automated Dielectric Single Cell Spectroscopy - Temperature Dependence of Electrorotation,” J. Phys. D: Appl. Phys. 35 12581270 (2002).Google Scholar
7. Ashkin, A., Dziedzic, J. M., Yamane, T., “Optical Trapping and Manipulation of Single Cells Using Infrared Laser Beams,” Nature 330 769771(1987).Google Scholar
8. Ashkin, A., Schutze, K., Dziedzic, J. M., Euteneuer, U. and Schliwa, M., “Force Generation of Organelle Transport In Vivo Measured by an Infrared Laser Trap,” Nature 348 346348 (1990).Google Scholar
9. Berns, M. W., Aist, J. R., Wright, W. H. and Liang, H., “Optical Trapping in Animal and Fungal Cells Using a Tunable, Near-infrared Titanium-sapphire Laser,” Exp. Cell Res. 198 375378 (1990).Google Scholar
10. Shivashankar, G. V., Stolovitzky, G. and Libchaber, A., “Backscattering From a Tethered Bead as a Probe of DNA Flexibility,” Appl. Phys. Lett. , 73, 291293 (1998).Google Scholar
11. Ghislain, L. P., Switz, N. A. and Webb, W. W., “Measurement of Small Forces Using on Optical Trap,” Rev. sci. instrum. 65 27622768 (1994).Google Scholar
12. Guo, H. L., Yao, X. C., Li, Z. L., Cheng, B. Y., Han, X. H. and Zhang, D. Z., Sci. in Chin. 45 919(2002).Google Scholar