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India Ink as a Tracer for Circulatory System Study in a Ganglion, With a Note on Using Ferritin, Lanthanum, and Horseradish Peroxidasefor the Blood-Brain Barrier

Published online by Cambridge University Press:  14 March 2018

JoAnn Buchanan
JoAnn Buchanan*
Affiliation:
Stanford University
*
redhair@leland.Stanford.edu

Extract

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Several summers ago, while working at the Marine Biological Laboratory in Woods Hole, Massachusetts, George Augustine (Duke University) and I wished to look at the circulation in the giant synapse of the squid, Loligo pealei. At that time, Dr. Eugene Copeland suggested the use of India ink in order to see the circulatory system. The ink contains carbon particles that are visible under the EM. In addition, it is visible to the naked eye.

Type
Research Article
Information
Microscopy Today , Volume 8 , Issue 1 , January 2000 , pp. 17
Copyright
Copyright © Microscopy Society of America 2000

References

Sanchez, M. E., C. M., Nuno, J., Buchanan, and Augustine, G. J.. 1990. Contractions of the squid stellate ganglion. J. Exp. Biol. 152, 369-387. (We were able to identify the blood vessels based on our previous work with the tracers.)CrossRefGoogle ScholarPubMed