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Detection of Ophiostoma Piceae in Radiata Pine using Immunofluorescence Labeling and Confocal Laser Scanning Microscopy

Published online by Cambridge University Press:  14 March 2018

Ying Xiao ,
Bernhard Kreber  and
Colette Breuil
Ying Xiao
Affiliation:
New Zealand Forest Research Institute Limited, Rotorua, New Zealand
Bernhard Kreber
Affiliation:
New Zealand Forest Research Institute Limited, Rotorua, New Zealand
Colette Breuil
Affiliation:
Faculty of Forestry, University of British Columbia, Vancouver, Canada

Extract

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The use of fluorescence microscopy to investigate fungal growth in wood often causes interference due to the strong autofluorescence of wood lignin, unless fluorescent probes which specifically react to fungal hyphae, are used. Techniques to enable differentiation of hyphae from wood have been recently reported (Singh et al., 1997; Xiao et al., 1997). The authors demonstrated that while glutaraldehyde can be used to detect fungal native proteins, wheat germ agglutinin (WGA) which reacts with cell wall chitin of hyphae, considerably improved detection of fungi growing in wood.

Type
Research Article
Information
Microscopy Today , Volume 6 , Issue 3 , April 1998 , pp. 24 - 25
Copyright
Copyright © Microscopy Society of America 1998

References

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