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Using MicroCT to evaluate stain penetration and establish fiducial marks for high throughput electron microscopy.

Published online by Cambridge University Press:  05 August 2019

JoAnn Buchanan*
Affiliation:
Allen Institute for Brain Science. Seattle, WA, USA.
Marc M. Takeno
Affiliation:
Allen Institute for Brain Science. Seattle, WA, USA.
Agnes L. Bodor
Affiliation:
Allen Institute for Brain Science. Seattle, WA, USA.
Daniel J. Bumbarger
Affiliation:
Allen Institute for Brain Science. Seattle, WA, USA.
E. Froudarakis
Affiliation:
Baylor College of Medicine, Dept. of Neuroscience, Houston, TX, USA.
Jacob Reimer
Affiliation:
Baylor College of Medicine, Dept. of Neuroscience, Houston, TX, USA.
Andres S. Tolias
Affiliation:
Baylor College of Medicine, Dept. of Neuroscience, Houston, TX, USA.
R. Clay Reid
Affiliation:
Allen Institute for Brain Science. Seattle, WA, USA.
Nuno M. da Costa
Affiliation:
Allen Institute for Brain Science. Seattle, WA, USA.
*
*Corresponding author: buchsmith@gmail.com
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Abstract

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Type
Multi-Modal, Large-Scale and 3D Correlative Microscopy
Copyright
Copyright © Microscopy Society of America 2019 

References

[1]Gray, EG Nature 183 (1959), p1592.CrossRefGoogle Scholar
[2]Dempster, W, American Journal of Anatomy 107 (1960) p59.CrossRefGoogle Scholar
[3]Tapia, J et al. , Nature Protocols 7 (2012), p193.CrossRefGoogle Scholar
[4]Hua, Y, Laserstein, P and Helmstaedter, M, Nature Communications 6 (2015) p1.Google Scholar
[5]Scheller, E et al. , Methods in enzymology 537 (2014) p123.CrossRefGoogle Scholar
[6]Imaging data was acquired through the Cornell University Biotechnology Resource Center, with NIH 1S10OD012287 funding for the ZEISS-Xradia Versa 520 X-ray Microscope.Google Scholar
[7]Supported by the Intelligence Advanced Research Projects Activity (IARPA) via Department of Interior/ Interior Business Center (DoI/IBC) contract number D16PC00004. The U.S. Government is authorized to reproduce and distribute reprints for Governmental purposes notwithstanding any copyright annotation thereon. Disclaimer: The views and conclusions contained herein are those of the authors and should not be interpreted as necessarily representing the official policies or endorsements, either expressed or implied, of IARPA, DoI/IBC, or the U.S. GovernmentGoogle Scholar
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Using MicroCT to evaluate stain penetration and establish fiducial marks for high throughput electron microscopy.
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