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Toxicity Evaluation of Two Dental Composites: Three-Dimensional Confocal Laser Scanning Microscopy Time-Lapse Imaging of Cell Behavior

Published online by Cambridge University Press:  02 May 2013

Ghania Nina Attik ,
Nelly Pradelle-Plasse ,
Doris Campos ,
Pierre Colon  and
Brigitte Grosgogeat
Ghania Nina Attik*
Affiliation:
Laboratoire des Multimatériaux et Interfaces, UMR CNRS 5615, Université Lyon1, Villeurbanne, France
Nelly Pradelle-Plasse
Affiliation:
UFR d'Odontologie, Université Paris Diderot, APHP, Hôpital Rothschild, Service d'Odontologie, Paris, France
Doris Campos
Affiliation:
Laboratoire des Multimatériaux et Interfaces, UMR CNRS 5615, Université Lyon1, Villeurbanne, France
Pierre Colon
Affiliation:
UFR d'Odontologie, Université Paris Diderot, APHP, Hôpital Rothschild, Service d'Odontologie, Paris, France
Brigitte Grosgogeat
Affiliation:
Laboratoire des Multimatériaux et Interfaces, UMR CNRS 5615, Université Lyon1, Villeurbanne, France UFR d'Odontologie, Université Lyon1, Service de Consultations et de Traitements Dentaires, Hospices Civils de Lyon, Lyon, France
*
*Corresponding author. E-mail: nina.attik@univ-lyon1.fr
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Abstract

The purpose of this study was to investigate the in vitro biocompatibility of two dental composites (namely A and B) with similar chemical composition used for direct restoration using three-dimensional confocal laser scanning microscopy (CLSM) time-lapse imaging. Time-lapse imaging was performed on cultured human HGF-1 fibroblast-like cells after staining using Live/Dead®. Image analysis showed a higher mortality rate in the presence of composite A than composite B. The viability rate decreased in a time-dependent manner during the 5 h of exposure. Morphological alterations were associated with toxic effects; cells were enlarged and more rounded in the presence of composite A as shown by F-actin and cell nuclei staining. Resazurin assay was used to confirm the active potential of composites in cell metabolism; results showed severe cytotoxic effects in the presence of both no light-curing composites after 24 h of direct contact. However, extracts of polymerized composites induced a moderate decrease in cell metabolism after the same incubation period. Composite B was significantly better tolerated than composite A at all investigated end points and all time points. The finding confirmed that the used CLSM method was sufficiently sensitive to differentiate the biocompatibility behavior of two composites based on similar methacrylate monomers.

Keywords

biocompatibilitycultured cellsLive/Dead® stainingscanning confocal microscopytime-lapse imagingdental compositeslight-curing timeviability
Type
Biological Applications
Information
Microscopy and Microanalysis , Volume 19 , Issue 3 , June 2013 , pp. 596 - 607
Copyright
Copyright © Microscopy Society of America 2013 

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