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A Tissue Engineering Model for Tractional Organization of Cells and Matrices

Published online by Cambridge University Press:  02 July 2020

Rajendra S. Bhatnagar ,
Steven B. Nicoll ,
Jing Jing Qian  and
Nancy Smith
Rajendra S. Bhatnagar
Affiliation:
Box 0424, S-512 University of California, San Francisco, CA, 94143-0424
Steven B. Nicoll
Affiliation:
Box 0424, S-512 University of California, San Francisco, CA, 94143-0424
Jing Jing Qian
Affiliation:
Box 0424, S-512 University of California, San Francisco, CA, 94143-0424
Nancy Smith
Affiliation:
Box 0424, S-512 University of California, San Francisco, CA, 94143-0424
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Abstract

Tissues are characterized by highly distinctive microarchitecture and overall form that are critical to tissue specific function. with increasing interest in tissue engineering to create surrogates for human tissues and organs, innovative techniques have been developed to generate scaffolds that display form, and microarchitecture mimicking physiological structures. While this artifice may generate pre-programmed shapes, it fails to take advantage of the inherent ability of cells to organize themselves and their surroundings. The generation of mechanical forces by the cellular cytoskeleton plays a critical role in the organization of matrix and of cellular colonies. The anchorage of the cytoskeleton to a substrate is essential for cellular tractional processes. in tissues, cells are anchored in a stationary, 3-D network of collagen in a highly characteristic spatial arrangement. This spatial order arises from vectorial deposition of matrix and its subsequent tractional organization by cells. The junction of the cell binding domain of collagen with specific integrin receptors, and the cells’ cytoskeleton comprises the apparatus for the interconversion of mechanical and biochemical energy.

Type
Advances in Imaging Techniques for Biomaterlals (Organized by S. Eppel)
Information
Microscopy and Microanalysis , Volume 7 , Issue S2: Proceedings: Microscopy & Microanalysis 2001, Microscopy Society of America 59th Annual Meeting, Microbeam Analysis Society 35th Annual Meeting, Long Beach, California August 5-9, 2001 , August 2001 , pp. 122 - 123
Copyright
Copyright © Microscopy Society of America 2001

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References

references

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