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Streptavidin Monolayer-Crystal Affinity Grids: A Step Toward Controlling What Happens During Cryo-EM Sample Preparation

Published online by Cambridge University Press:  04 August 2017

Robert M. Glaeser
Affiliation:
Molecular Biophysics and Integrated Bioimaging Division, Lawrence Berkeley National Laboratory, University of California, Berkeley, CA, USA
Bong-Gyoon Han
Affiliation:
Molecular Biophysics and Integrated Bioimaging Division, Lawrence Berkeley National Laboratory, University of California, Berkeley, CA, USA
Jamie H. D. Cate
Affiliation:
Molecular Biophysics and Integrated Bioimaging Division, Lawrence Berkeley National Laboratory, University of California, Berkeley, CA, USA Department of Molecular and Cell Biology, California Institute of Quantitative Biosciences, and Department of Chemistry, University of California, Berkeley, CA, USA
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Abstract

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© Microscopy Society of America 2017 

References

[1] Glaeser, RM & Han, BG Biophysics Reports (2016) p.1.Google Scholar
[2] Yoshimura, H, Scheybani, T & Baumeister, W Langmuir 10 1994). p. 3290.CrossRefGoogle Scholar
[3] Han, BG, et al, J Struct Biol 195 2016). p. 238.CrossRefGoogle Scholar
[4] The authors acknowledge funding from NIH grants P01 GM051487 and R01 GM065050. We thank Dr. Arto Pulk for providing the sample of randomly biotinylated 70S ribosome particles.Google Scholar
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Streptavidin Monolayer-Crystal Affinity Grids: A Step Toward Controlling What Happens During Cryo-EM Sample Preparation
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