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Quantitative Studies of Endothelial Cell Fibronectin and Filamentous Actin (F-Actin) Coalignment in Response to Shear Stress

Published online by Cambridge University Press:  12 September 2017

Xianghui Gong ,
Xixi Zhao ,
Bin Li ,
Yan Sun ,
Meili Liu ,
Yan Huang ,
Xiaoling Jia ,
Jing Ji  and
Yubo Fan
Xianghui Gong*
Affiliation:
Key Laboratory for Biomechanics and Mechanobiology of Ministry of Education, School of Biological Science and Medical Engineering, Beihang University, Beijing 100191, People’s Republic of China
Xixi Zhao
Affiliation:
Key Laboratory for Biomechanics and Mechanobiology of Ministry of Education, School of Biological Science and Medical Engineering, Beihang University, Beijing 100191, People’s Republic of China
Bin Li
Affiliation:
Key Laboratory for Biomechanics and Mechanobiology of Ministry of Education, School of Biological Science and Medical Engineering, Beihang University, Beijing 100191, People’s Republic of China
Yan Sun
Affiliation:
Key Laboratory for Biomechanics and Mechanobiology of Ministry of Education, School of Biological Science and Medical Engineering, Beihang University, Beijing 100191, People’s Republic of China
Meili Liu
Affiliation:
Key Laboratory for Biomechanics and Mechanobiology of Ministry of Education, School of Biological Science and Medical Engineering, Beihang University, Beijing 100191, People’s Republic of China
Yan Huang
Affiliation:
Key Laboratory for Biomechanics and Mechanobiology of Ministry of Education, School of Biological Science and Medical Engineering, Beihang University, Beijing 100191, People’s Republic of China
Xiaoling Jia
Affiliation:
Key Laboratory for Biomechanics and Mechanobiology of Ministry of Education, School of Biological Science and Medical Engineering, Beihang University, Beijing 100191, People’s Republic of China
Jing Ji
Affiliation:
Key Laboratory for Biomechanics and Mechanobiology of Ministry of Education, School of Biological Science and Medical Engineering, Beihang University, Beijing 100191, People’s Republic of China
Yubo Fan*
Affiliation:
Key Laboratory for Biomechanics and Mechanobiology of Ministry of Education, School of Biological Science and Medical Engineering, Beihang University, Beijing 100191, People’s Republic of China National Research Center for Rehabilitation Technical Aids, Beijing 100176, People’s Republic of China
*
*Corresponding authors. xhgong@buaa.edu.cn; yubofan@buaa.edu.cn
*Corresponding authors. xhgong@buaa.edu.cn; yubofan@buaa.edu.cn
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Abstract

Both fibronectin (FN) and filamentous actin (F-actin) fibers play a critical role for endothelial cells (ECs) in responding to shear stress and modulating cell alignment and functions. FN is dynamically coupled to the F-actin cytoskeleton via focal adhesions. However, it is unclear how ECs cooperatively remodel their subcellular FN matrix and intracellular F-actin cytoskeleton in response to shear stress. Current studies are hampered by the lack of a reliable and sensitive quantification method of FN orientation. In this study, we developed a MATLAB-based feature enhancement method to quantify FN and F-actin orientation. The role of F-actin in FN remodeling was also studied by treating ECs with cytochalasin D. We have demonstrated that FN and F-actin codistributed and coaligned parallel to the flow direction, and that F-actin alignment played an essential role in regulating FN alignment in response to shear stress. Our findings offer insight into how ECs cooperatively remodel their subcellular ECM and intracellular F-actin cytoskeleton in response to mechanical stimuli, and are valuable for vascular tissue engineering.

Keywords

fibronectinF-actinendothelial cellcoalignmentshear stress
Type
Biological Science Applications
Information
Microscopy and Microanalysis , Volume 23 , Issue 5 , October 2017 , pp. 1013 - 1023
Copyright
© Microscopy Society of America 2017 

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Footnotes

a

Xianghui Gong and Xixi Zhao contributed equally to this work.

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