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Protocol Development for 3D Reconstructions: Combining In Vivo Imaging, APEX2 and En Bloc Staining of Mouse Visual Cortex

Published online by Cambridge University Press:  23 September 2015

JoAnn Buchanan
Affiliation:
Allen Institute for Brain Science, Seattle, WA, USA
Marc Takeno
Affiliation:
Allen Institute for Brain Science, Seattle, WA, USA
Tanya Daigle
Affiliation:
Allen Institute for Brain Science, Seattle, WA, USA
Adam Bleckert
Affiliation:
Allen Institute for Brain Science, Seattle, WA, USA
Daniel Bumbarger
Affiliation:
Allen Institute for Brain Science, Seattle, WA, USA
Agnes Bodor
Affiliation:
Allen Institute for Brain Science, Seattle, WA, USA
Derrick Brittain
Affiliation:
Allen Institute for Brain Science, Seattle, WA, USA
Ali Cetin
Affiliation:
Allen Institute for Brain Science, Seattle, WA, USA
Nuno Macarico da Costa
Affiliation:
Allen Institute for Brain Science, Seattle, WA, USA

Abstract

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Copyright
Copyright © Microscopy Society of America 2015 

References

[1] Lam, S., et al., Nature Methods 12 (2015). p 51.CrossRefGoogle Scholar
[2] Martell, J., et al., Nature Biotechnology 30 (2012). p 1243.CrossRefGoogle Scholar
[3] Mikula, S., Binding, J. & Denk, W., Nature Methods 9 (2012). p 1198.CrossRefGoogle Scholar
[4] The authors are grateful to the Allen Institute founders, P.G. Allen and J. Allen, for their vision, encouragement, and support..Google Scholar
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Protocol Development for 3D Reconstructions: Combining In Vivo Imaging, APEX2 and En Bloc Staining of Mouse Visual Cortex
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