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Multiscale 3D Virtual Dissections of 100-Million-Year-Old Flowers Using X-Ray Synchrotron Micro- and Nanotomography

Published online by Cambridge University Press:  07 January 2014

Jean-David Moreau ,
Peter Cloetens ,
Bernard Gomez ,
Véronique Daviero-Gomez ,
Didier Néraudeau ,
Tamzin A. Lafford  and
Paul Tafforeau
Jean-David Moreau*
Affiliation:
Université Rennes 1, CNRS UMR 6118 Géosciences Rennes, Campus de Beaulieu bât. 15, 263 avenue du Général Leclerc, 35042 Rennes, France European Synchrotron Radiation Facility, 6, rue J.-Horowitz, BP 220, 38043 Grenoble, France
Peter Cloetens
Affiliation:
European Synchrotron Radiation Facility, 6, rue J.-Horowitz, BP 220, 38043 Grenoble, France
Bernard Gomez
Affiliation:
Université Lyon 1 (Claude Bernard), CNRS UMR 5276 LGL-TPE, OSU Lyon, Campus La Doua, Bât. Géode, 43 boulevard du 11 Novembre 1918, 69622 Villeurbanne, France
Véronique Daviero-Gomez
Affiliation:
Université Lyon 1 (Claude Bernard), CNRS UMR 5276 LGL-TPE, OSU Lyon, Campus La Doua, Bât. Géode, 43 boulevard du 11 Novembre 1918, 69622 Villeurbanne, France
Didier Néraudeau
Affiliation:
Université Rennes 1, CNRS UMR 6118 Géosciences Rennes, Campus de Beaulieu bât. 15, 263 avenue du Général Leclerc, 35042 Rennes, France
Tamzin A. Lafford
Affiliation:
European Synchrotron Radiation Facility, 6, rue J.-Horowitz, BP 220, 38043 Grenoble, France
Paul Tafforeau
Affiliation:
European Synchrotron Radiation Facility, 6, rue J.-Horowitz, BP 220, 38043 Grenoble, France
*
*Corresponding author. E-mail: jean.david.moreau@gmail.com
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Abstract

A multiscale approach combining phase-contrast X-ray micro- and nanotomography is applied for imaging a Cretaceous fossil inflorescence in the resolution range from 0.75 μm to 50 nm. The wide range of scale views provides three-dimensional reconstructions from the external gross morphology of the inflorescence fragment to the finest exine sculptures of in situ pollen. This approach enables most of the characteristics usually observed under light microscopy, or with low magnification under scanning and transmission electron microscopy, to be obtained nondestructively. In contrast to previous tomography studies of fossil and extant flowers that used resolutions down to the micron range, we used voxels with a 50 nm side in local tomography scans. This high level of resolution enables systematic affinities of fossil flowers to be established without breaking or slicing specimens.

Keywords

propagation phase-contrast tomographymicrotomographynanotomographymultiscale approachfossil plantsflowersCretaceous
Type
Biological Applications
Information
Microscopy and Microanalysis , Volume 20 , Issue 1 , February 2014 , pp. 305 - 312
Copyright
Copyright © Microscopy Society of America 2014 

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