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Morphological and Histochemical Changes in Macrophage Activity After Novel Anti-Neoplastic Platinum Agents

Published online by Cambridge University Press:  02 July 2020

H. J. Muenchen ,
S.K. Aggarwal ,
H.K. Misra  and
P. J. Andrulis
H. J. Muenchen
Affiliation:
Department of Zoology, Michigan State University, East Lansing, MI, 48824-1115
S.K. Aggarwal
Affiliation:
Department of Zoology, Michigan State University, East Lansing, MI, 48824-1115
H.K. Misra
Affiliation:
Andrulis Pharmaceuticals Corporation, Beltsville, Maryland, 20705
P. J. Andrulis
Affiliation:
Andrulis Pharmaceuticals Corporation, Beltsville, Maryland, 20705
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Extract

Poly-[(trans-1,2-diaminocyclohexane) platinumj-carboxyamylose (“poly-plat”), 5-sulfosalicylato-trans -(1,2-diaminocyclohexane) platinum (SSP), and 4-hydroxy-∝-sulfonylphenylacetato (trans 1,2-diaminocyclohexane) platinum (II) (SAP) are second generation analogs of cisplatin (CDDP) with higher efficacy and potency than cisplatin. This is particularly true of “poly-plat” which contains 1/5 the platinum of CDDP. In order to understand the mechanism of action of these compounds, isolated murine peritoneal macrophages in culture medium were treated with “poly-plat”, SSP, or SAP (5 μg/ml) for 2 h. Drug containing medium was then replaced with fresh medium and the cells were allowed to incubate at 37° C (5% CO2) for 24 h. Supernatants were collected at 0.5, 1, 2, and 24 h post-treatment for immunocytochemical analysis. Confocal microscopy studies demonstrated an increase in the number of lysosomes in the treated macrophages, but only “poly-plat” and SSP treated macrophages were stimulated to form cytoplasmic extensions at 2 h and 24 h.

Type
Neoplasia: Abnormal Cell Growth or Death/Apoptosis? Insights from Microscopy
Information
Microscopy and Microanalysis , Volume 3 , Issue S2: Proceedings: Microscopy & Microanalysis '97, Microscopy Society of America 55th Annual Meeting, Microbeam Analysis Society 31st Annual Meeting, Histochemical Society 48th Annual Meeting, Cleveland, Ohio, August 10-14, 1997 , August 1997 , pp. 11 - 12
Copyright
Copyright © Microscopy Society of America 1997

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References

1. Fiebig, H.H.et al., GB-21, a novel platinum polymer complex with antitumor activity in human renal and mammary xenografts. Proc. Amer. Cancer Res. 37(1996)297.Google Scholar

2. Palma, J.P. and Aggarwal, S.K., Cisplatin and carboplatin mediated release of cytolytic factors in murine peritoneal macrophages in vitro. Anti-cancer Drugs 3(1992)665.CrossRefGoogle Scholar