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Morphologic Identification of Viral Gastroenteritis Caused by a Human Calicivirus Strain Undetected Initially by Molecular or Immunologic Methods

Published online by Cambridge University Press:  02 July 2020

C. D. Humphrey ,
J. S. Noel ,
B. L. Liu ,
E. M. Rodriguez ,
P. R. Lambden ,
I. N. Clarke ,
D. M. Dwyer ,
T. Ando ,
R.I. Glass  and
S. S. Monroe
C. D. Humphrey
Affiliation:
Molecular Pathology and Ultrastructure; Atlanta, Ga30333
J. S. Noel
Affiliation:
Viral Gastroenteritis Section, DVRD; Atlanta, Ga30333
B. L. Liu
Affiliation:
Molecular Microbiology Group, Univ. Med Sch. Southampton General Hospital, SouthamptonUK;
E. M. Rodriguez
Affiliation:
Epidemic Intelligence Service, CDC, Atlanta, Ga30333; Maryland Dept. of Health & Mental Hygiene, Epidemiology and Disease Control, Baltimore, Md21201
P. R. Lambden
Affiliation:
Molecular Microbiology Group, Univ. Med Sch. Southampton General Hospital, SouthamptonUK;
I. N. Clarke
Affiliation:
Molecular Microbiology Group, Univ. Med Sch. Southampton General Hospital, SouthamptonUK;
D. M. Dwyer
Affiliation:
Maryland Dept. of Health & Mental Hygiene, Epidemiology and Disease Control, Baltimore, Md21201
T. Ando
Affiliation:
Viral Gastroenteritis Section, DVRD; Atlanta, Ga30333
R.I. Glass
Affiliation:
Viral Gastroenteritis Section, DVRD; Atlanta, Ga30333
S. S. Monroe
Affiliation:
Viral Gastroenteritis Section, DVRD; Atlanta, Ga30333
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Extract

Molecular and immunologie assays for virus identification and characterization are well known for their high specificity and sensitivity. High specificity occasionally hampers efforts to detect virus strains distantly related to previously recognized types and may allow infectious agents to be undetected when these techniques are used for identification. We describe in this report such an example with an outbreak of foodborne gastroenteritis.

Forty-six cases of gastrointestinal illness were reported in May 1994 among adult staff of a Parkville, Md., school after a catered luncheon. Symptoms included stomach cramps, nausea, diarrhea, and vomiting. Seven stool specimens, negative by routine bacteriological screening, and 14 acute-and convalescent-phase sera from patients involved in the outbreak were tested for viruses. The stool specimens were examined by electron microscopy (EM) and reverse transcription-polymerase chain reaction (RT-PCR) designed to detect small round structured viruses (SRSVs). In addition, serum specimens were tested for IgG antibody to recombinant capsid proteins from Norwalk virus (rNV) and Toronto virus (rTV) using a direct enzyme immunoassay (EIA).

Type
Pathology
Information
Microscopy and Microanalysis , Volume 3 , Issue S2: Proceedings: Microscopy & Microanalysis '97, Microscopy Society of America 55th Annual Meeting, Microbeam Analysis Society 31st Annual Meeting, Histochemical Society 48th Annual Meeting, Cleveland, Ohio, August 10-14, 1997 , August 1997 , pp. 79 - 80
Copyright
Copyright © Microscopy Society of America 1997

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