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Cryo-ET Characterization of Novel Cellular Extrusions in Escherichia coli Induced by the Major Subunit Protein of Type IV Pili, PilA, from Pseudomonas aeruginosa

Published online by Cambridge University Press:  30 July 2021

Juan Sanchez
Affiliation:
Biophysics Program, University of Wisconsin – Madison, Madison, Wisconsin, United States
Daniel Parrell
Affiliation:
Department of Biochemistry, University of Wisconsin – Madison, Madison, Wisconsin, United States
Alba Gonzalez-Rivera
Affiliation:
Department of Bacteriology, University of Wisconsin – Madison, Madison, Wisconsin, United States
Nicoleta Ploscariu
Affiliation:
Department of Biochemistry, University of Wisconsin – Madison, Madison, Wisconsin, United States
Katrina Forest
Affiliation:
Department of Bacteriology, University of Wisconsin – Madison, Madison, Wisconsin, United States
Elizabeth R. Wright
Affiliation:
Department of Biochemistry, University of Wisconsin – Madison, Madison, Wisconsin, United States Cryo-Electron Microscopy Research Center, Midwest Center for Cryo-Electron Tomography, University of Wisconsin – Madison, Madison, Wisconsin, United States Morgridge Institute for Research, University of Wisconsin – Madison, Madison, Wisconsin, United States

Abstract

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Type
3D Structures: From Macromolecular Assemblies to Whole Cells (3DEM FIG)
Copyright
Copyright © The Author(s), 2021. Published by Cambridge University Press on behalf of the Microscopy Society of America

References

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This research was supported by funds from the University of Wisconsin-Madison, National Institutes of Health (R01GM104540 and R01GM104540-03S1) to E.R.W. J.C.S. was supported in part by the Biotechnology Training Program, T32GM135066. All EM data was collected at the University of Wisconsin-Madison, Department of Biochemistry Cryo-EM Research Center. The authors gratefully acknowledge use of facilities and instrumentation at the UW-Madison Wisconsin Centers for Nanoscale Technology (wcnt.wisc.edu) partially supported by the NSF through the University of Wisconsin Materials Research Science and Engineering Center (DMR-1720415).Google Scholar