Hostname: page-component-848d4c4894-nr4z6 Total loading time: 0 Render date: 2024-05-13T12:32:33.310Z Has data issue: false hasContentIssue false

CryoEM Map-Model Scores: From Average Density to Q-scores

Published online by Cambridge University Press:  30 July 2021

Grigore Pintilie
Affiliation:
Department of Bioengineering, James H. Clark Center, Stanford University, Stanford, California, 94305 USA, United States
Wah Chiu
Affiliation:
School of Medicine, Stanford University, Stanford, California, United States

Abstract

Image of the first page of this content. For PDF version, please use the ‘Save PDF’ preceeding this image.'
Type
Michael Rossmann Memorial Symposium
Copyright
Copyright © The Author(s), 2021. Published by Cambridge University Press on behalf of the Microscopy Society of America

References

Rossmann, M. G., “Fitting atomic models into electron-microscopy maps,” Acta Crystallogr. D Biol. Crystallogr., vol. 56, no. Pt 10, pp. 13411349, Oct. 2000.CrossRefGoogle ScholarPubMed
Rossmann, M. G., Bernal, R., and Pletnev, S. V., “Combining electron microscopic with x-ray crystallographic structures,” J. Struct. Biol., vol. 136, no. 3, pp. 190200, Dec. 2001, doi: 10.1006/jsbi.2002.4435.CrossRefGoogle ScholarPubMed
Lagerstedt, I. et al. , “Web-based visualisation and analysis of 3D electron-microscopy data from EMDB and PDB,” J. Struct. Biol., vol. 184, no. 2, pp. 173181, Nov. 2013, doi: 10.1016/j.jsb.2013.09.021.CrossRefGoogle ScholarPubMed
Goddard, T. D., Huang, C. C., and Ferrin, T. E., “Visualizing density maps with UCSF Chimera,” J. Struct. Biol., vol. 157, no. 1, pp. 281287, Jan. 2007, doi: 10.1016/j.jsb.2006.06.010.Google ScholarPubMed
Stewart, P. L., Fuller, S. D., and Burnett, R. M., “Difference imaging of adenovirus: bridging the resolution gap between X-ray crystallography and electron microscopy,” EMBO J., vol. 12, no. 7, pp. 25892599, Jul. 1993.CrossRefGoogle ScholarPubMed
Pintilie, G., Zhang, K., Su, Z., Li, S., Schmid, M. F., and Chiu, W., “Measurement of atom resolvability in cryo-EM maps with Q -scores,” Nat. Methods, pp. 17, Feb. 2020, doi: 10.1038/s41592-020-0731-1.Google ScholarPubMed
Lawson, C. L. et al. , “Cryo-EM model validation recommendations based on outcomes of the 2019 EMDataResource challenge,” Nat. Methods, vol. 18, no. 2, Art. no. 2, Feb. 2021, doi: 10.1038/s41592-020-01051-w.CrossRefGoogle ScholarPubMed
Pintilie, Grigore, MapQ, DOI: 10.5281/zenodo.3595455 https://github.com/gregdp/mapq. 2019.CrossRefGoogle Scholar