Hostname: page-component-848d4c4894-nmvwc Total loading time: 0 Render date: 2024-06-23T07:50:53.859Z Has data issue: false hasContentIssue false

Cryo-Electron Tomography of Microtubules and Granules in Mouse Platelets

Published online by Cambridge University Press:  30 July 2021

Juleen Dickson
Affiliation:
University of Wisconsin Madison, Madison, Wisconsin, United States
Wenchun Chen
Affiliation:
Emory University School of Medicine, Atlanta, Georgia, United States
Joshua Strauss
Affiliation:
University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, United States
Renhao Li
Affiliation:
Emory University School of Medicine, Atlanta, Georgia, United States
Elizabeth Wright
Affiliation:
Department of Biochemistry, University of Wisconsin-Madison, Madison, Wisconsin, United States

Abstract

Image of the first page of this content. For PDF version, please use the ‘Save PDF’ preceeding this image.'
Type
3D Structures: From Macromolecular Assemblies to Whole Cells (3DEM FIG)
Copyright
Copyright © The Author(s), 2021. Published by Cambridge University Press on behalf of the Microscopy Society of America

References

Shin, E.-K., et al. , Platelet Shape Changes and Cytoskeleton Dynamics as Novel Therapeutic Targets for Anti-Thrombotic Drugs. Biomolecules & therapeutics, 2017. 25(3): p. 223-230.CrossRefGoogle ScholarPubMed
Blair, P. and Flaumenhaft, R., Platelet alpha-granules: basic biology and clinical correlates. Blood Rev, 2009. 23(4): p. 177-89.Google ScholarPubMed
Hartwig, J.H. and DeSisto, M., The cytoskeleton of the resting human blood platelet: structure of the membrane skeleton and its attachment to actin filaments. J Cell Biol, 1991. 112(3): p. 407-25.CrossRefGoogle ScholarPubMed
Woronowicz, K., et al. , The Platelet Actin Cytoskeleton Associates with SNAREs and Participates in α-Granule Secretion. Biochemistry, 2010. 49(21): p. 4533-4542.CrossRefGoogle ScholarPubMed
Mastronarde, D., Automated electron microscope tomography using robust prediction of specimen movements. Journal of structural biology, 2005. 152: p. 36-51.Google ScholarPubMed
Kremer, J.R., Mastronarde, D.N., and McIntosh, J.R., Computer visualization of three-dimensional image data using IMOD. J Struct Biol, 1996. 116(1): p. 71-6.CrossRefGoogle ScholarPubMed
Tang, G., et al. , EMAN2: an extensible image processing suite for electron microscopy. J Struct Biol, 2007. 157(1): p. 38-46.Google ScholarPubMed