Hostname: page-component-78c5997874-94fs2 Total loading time: 0 Render date: 2024-11-19T13:19:40.895Z Has data issue: false hasContentIssue false
  • English
  • Français

Computational Recovery of Engineered Point Spread Functions in Single Molecule Localization Microscopy using the Double Helix 3DTRAX Software

Published online by Cambridge University Press:  30 July 2021

Scott Gaumer ,
Warren Colomb ,
Anjul Loiacono ,
Leslie Kimerling  and
Anurag Agrawal
Scott Gaumer
Affiliation:
Double Helix Optics, United States
Warren Colomb
Affiliation:
Double Helix Optics, United States
Anjul Loiacono
Affiliation:
Double Helix Optics, United States
Leslie Kimerling
Affiliation:
Double Helix Optics, Boulder, Colorado, United States
Anurag Agrawal
Affiliation:
Double Helix Optics, United States

Abstract

An abstract is not available for this content so a preview has been provided. As you have access to this content, a full PDF is available via the ‘Save PDF’ action button.
Image of the first page of this content. For PDF version, please use the ‘Save PDF’ preceeding this image.'
Type
Frontiers in Fluorescence Lifetime and Super-resolution Imaging of Biological Structures and Dynamics
Information
Microscopy and Microanalysis , Volume 27 , Supplement S1 , August 2021 , pp. 868 - 871
Copyright
Copyright © The Author(s), 2021. Published by Cambridge University Press on behalf of the Microscopy Society of America

References

Rust, QM. J., et al. , “Sub-diffraction-limit imaging by stochastic optical reconstruction microscopy (STORM).,” Nat. Methods, vol. 3, no. 10, pp. 793–5, Oct. 2006.CrossRefGoogle ScholarPubMed
Pavani, S. R. P. et al. , “High-efficiency rotating point spread functions,” Opt. Express, vol. 16, no. 5, pp. 3484–3489, Mar. 2008.CrossRefGoogle Scholar
Grover, G., et al. , “Performance limits on three-dimensional particle localization in photon-limited microscopy,” Opt. Lett., vol. 35, no. 19, p. 3306, Sep. 2010.Google Scholar
Shechtman, Y., et al. , “Precise Three-Dimensional Scan-Free Multiple-Particle Tracking over Large Axial Ranges with Tetrapod Point Spread Functions,” Nano Lett., vol. 15, no. 6, pp. 4194–4199, Jun. 2015.CrossRefGoogle Scholar
Jain, S., et al. , “ATPase-Modulated Stress Granules Contain a Diverse Proteome and Substructure,” Cell, vol. 164, no. 3, pp. 487–498, 2016.Google Scholar
Wang, D., et al. , “Enhanced information content for three-dimensional localization and tracking using the double-helix point spread function with variable-angle illumination epifluorescence microscopy,” Appl. Phys. Lett., vol. 110, no. 21, p. 211107, May 2017.CrossRefGoogle Scholar
Gahlmann, A. et al. , “Quantitative multicolor subdiffraction imaging of bacterial protein ultrastructures in three dimensions.,” Nano Lett., vol. 13, no. 3, pp. 987–93, Mar. 2013.CrossRefGoogle ScholarPubMed
Pavani, S. R. P. et al. , “Three-dimensional, single-molecule fluorescence imaging beyond the diffraction limit by using a double-helix point spread function.,” Proc. Natl. Acad. Sci. U. S. A., vol. 106, no. 9, pp. 2995–9, Mar. 2009.CrossRefGoogle ScholarPubMed
Gustavsson, A.-K., et al. , “3D single-molecule super-resolution microscopy with a tilted light sheet,” Nat. Commun. 2018 91, vol. 9, no. 1, p. 123, Jan. 2018.Google Scholar
Shechtman, Y., et al. , “Multicolour localization microscopy by point-spread-function engineering,” Nat. Photonics, vol. 10, no. 9, pp. 590–594, Sep. 2016.Google Scholar
Lam, W. Y. et al. , “HER2 Cancer Protrusion Growth Signaling Regulated by Unhindered, Localized Filopodial Dynamics,” bioRxiv, p. 654988, Jun. 2019.Google Scholar